diff --git a/pkgs/development/python-modules/napari/default.nix b/pkgs/development/python-modules/napari/default.nix
index 93252b0e9109..8d3a0bf5b824 100644
--- a/pkgs/development/python-modules/napari/default.nix
+++ b/pkgs/development/python-modules/napari/default.nix
@@ -40,7 +40,7 @@
 
 mkDerivationWith buildPythonPackage rec {
   pname = "napari";
-  version = "0.4.19.post1";
+  version = "0.5.4";
   pyproject = true;
 
   disabled = pythonOlder "3.9";
@@ -49,11 +49,11 @@ mkDerivationWith buildPythonPackage rec {
     owner = "napari";
     repo = "napari";
     rev = "refs/tags/v${version}";
-    hash = "sha256-qw5WdFPySNkmm+dNu+hqsmr+csBpHnSl9bMpb4nKEqI=";
+    hash = "sha256-wJifLRrHlDzPgBU7OOPqjdzYpr9M+Klc+yAc/IpyZN8=";
   };
 
   postPatch = ''
-    substituteInPlace setup.cfg \
+    substituteInPlace pyproject.toml \
       --replace-fail "scikit-image[data]>=0.19.1" "scikit-image"
   '';