diff --git a/pkgs/misc/emulators/wxmupen64plus/default.nix b/pkgs/misc/emulators/wxmupen64plus/default.nix
deleted file mode 100644
index c09a83b92ea8..000000000000
--- a/pkgs/misc/emulators/wxmupen64plus/default.nix
+++ /dev/null
@@ -1,32 +0,0 @@
-{ lib, stdenv, fetchurl, python2, wxGTK29, mupen64plus, SDL, libX11, libGLU, libGL
-, wafHook }:
-
-stdenv.mkDerivation rec {
-  pname = "wxmupen64plus";
-  version = "0.3";
-
-  src = fetchurl {
-    url = "https://bitbucket.org/auria/wxmupen64plus/get/${version}.tar.bz2";
-    sha256 = "1mnxi4k011dd300k35li2p6x4wccwi6im21qz8dkznnz397ps67c";
-  };
-
-  nativeBuildInputs = [ wafHook ];
-  buildInputs = [ python2 wxGTK29 SDL libX11 libGLU libGL ];
-
-  preConfigure = ''
-    tar xf ${mupen64plus.src}
-    APIDIR=$(eval echo `pwd`/mupen64plus*/source/mupen64plus-core/src/api)
-    export CXXFLAGS="-I${libX11.dev}/include/X11 -DLIBDIR=\\\"${mupen64plus}/lib/\\\""
-    export LDFLAGS="-lwx_gtk2u_adv-2.9"
-
-    wafConfigureFlagsArray+=("--mupenapi=$APIDIR" "--wxconfig=`type -P wx-config`")
-  '';
-
-  NIX_CFLAGS_COMPILE = "-fpermissive";
-
-  meta = {
-    description = "GUI for the Mupen64Plus 2.0 emulator";
-    license = lib.licenses.gpl2Plus;
-    homepage = "https://bitbucket.org/auria/wxmupen64plus/wiki/Home";
-  };
-}
diff --git a/pkgs/top-level/aliases.nix b/pkgs/top-level/aliases.nix
index 646ad56d5d89..ab07fd2c9267 100644
--- a/pkgs/top-level/aliases.nix
+++ b/pkgs/top-level/aliases.nix
@@ -1098,6 +1098,7 @@ mapAliases ({
   winswitch = throw "winswitch has been removed from nixpkgs."; # added 2019-12-10
   wireshark-gtk = throw "wireshark-gtk is not supported anymore. Use wireshark-qt or wireshark-cli instead."; # added 2019-11-18
   wireguard = wireguard-tools; # added 2018-05-19
+  wxmupen64plus = throw "wxmupen64plus was removed because the upstream disappeared."; # 2022-01-31
   morituri = whipper; # added 2018-09-13
   xbmc-retroarch-advanced-launchers = kodi-retroarch-advanced-launchers; # added 2021-11-19
   xp-pen-g430 = pentablet-driver; # added 2020-05-03
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index b4d73f9c8b93..2573c7de47af 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -34124,8 +34124,6 @@ with pkgs;
 
   wraith = callPackage ../applications/networking/irc/wraith { };
 
-  wxmupen64plus = callPackage ../misc/emulators/wxmupen64plus { };
-
   wxsqlite3 = callPackage ../development/libraries/wxsqlite3 {
     wxGTK = wxGTK30;
     inherit (darwin.apple_sdk.frameworks) Cocoa;