diff --git a/pkgs/development/python-modules/mypy-boto3/default.nix b/pkgs/development/python-modules/mypy-boto3/default.nix
index 512c7ffa183e..e06f7ec73bc3 100644
--- a/pkgs/development/python-modules/mypy-boto3/default.nix
+++ b/pkgs/development/python-modules/mypy-boto3/default.nix
@@ -446,8 +446,8 @@ in
       "sha256-eKwS+FXicnxiHr2aTDG+ih6kDxQ24NLCkI9XPt2LjvQ=";
 
   mypy-boto3-ec2 =
-    buildMypyBoto3Package "ec2" "1.40.61"
-      "sha256-NfY+6/jTnWgQs8g+RSst9fqojvaXgC0Ulzd+VBlMq0E=";
+    buildMypyBoto3Package "ec2" "1.40.64"
+      "sha256-6+jjuG6iqXLjjuztY1FX/exSl5BjdKsrg1EOQM1w0Io=";
 
   mypy-boto3-ec2-instance-connect =
     buildMypyBoto3Package "ec2-instance-connect" "1.40.57"