diff --git a/pkgs/applications/science/biology/truvari/default.nix b/pkgs/applications/science/biology/truvari/default.nix
index 412642f523f2..37aa4e8789ab 100644
--- a/pkgs/applications/science/biology/truvari/default.nix
+++ b/pkgs/applications/science/biology/truvari/default.nix
@@ -1,45 +1,74 @@
 { lib
 , fetchFromGitHub
 , python3Packages
+, runtimeShell
+, bcftools
+, htslib
 }:
 
-python3Packages.buildPythonApplication rec {
+let
+  ssshtest = fetchFromGitHub {
+    owner = "ryanlayer";
+    repo = "ssshtest";
+    rev = "d21f7f928a167fca6e2eb31616673444d15e6fd0";
+    hash = "sha256-zecZHEnfhDtT44VMbHLHOhRtNsIMWeaBASupVXtmrks=";
+  };
+in python3Packages.buildPythonApplication rec {
   pname = "truvari";
-  version = "2.1.1";
+  version = "4.0.0";
 
   src = fetchFromGitHub {
-    owner = "spiralgenetics";
+    owner = "ACEnglish";
     repo = "truvari";
     rev = "v${version}";
-    sha256 = "14nsdbj063qm175xxixs34cihvsiskc9gym8pg7gbwsh13k5a00h";
+    hash = "sha256-UJNMKEV5m2jFqnWvkVAtymkcE2TjPIXp7JqRZpMSqsE=";
   };
 
   postPatch = ''
     substituteInPlace setup.py \
-      --replace 'python-Levenshtein==0.12.1' 'python-Levenshtein>=0.12.1'
+      --replace "rich==" "rich>="
+    substituteInPlace truvari/utils.py \
+      --replace "/bin/bash" "${runtimeShell}"
+    patchShebangs repo_utils/test_files
   '';
 
   propagatedBuildInputs = with python3Packages; [
-    pyvcf
-    levenshtein
-    progressbar2
+    rich
+    edlib
     pysam
-    pyfaidx
     intervaltree
-    pytabix
-    acebinf
-    bwapy
     joblib
+    numpy
+    pytabix
+    bwapy
     pandas
   ];
 
-  # no tests
-  doCheck = false;
+  makeWrapperArgs = [
+    "--prefix" "PATH" ":" (lib.makeBinPath [ bcftools htslib ])
+  ];
+
   pythonImportsCheck = [ "truvari" ];
 
+  nativeCheckInputs = [
+    bcftools
+    htslib
+  ] ++ (with python3Packages; [
+    coverage
+  ]);
+
+  checkPhase = ''
+    runHook preCheck
+
+    ln -s ${ssshtest}/ssshtest .
+    bash repo_utils/truvari_ssshtests.sh
+
+    runHook postCheck
+  '';
+
   meta = with lib; {
     description = "Structural variant comparison tool for VCFs";
-    homepage = "https://github.com/spiralgenetics/truvari";
+    homepage = "https://github.com/ACEnglish/truvari";
     license = licenses.mit;
     maintainers = with maintainers; [ scalavision ];
     longDescription = ''