diff --git a/nixos/doc/manual/from_md/release-notes/rl-2205.section.xml b/nixos/doc/manual/from_md/release-notes/rl-2205.section.xml
index 28a5d12201e4..0c10451add4c 100644
--- a/nixos/doc/manual/from_md/release-notes/rl-2205.section.xml
+++ b/nixos/doc/manual/from_md/release-notes/rl-2205.section.xml
@@ -766,6 +766,18 @@
           directly.
         </para>
       </listitem>
+      <listitem>
+        <para>
+          The <literal>dendrite</literal> package has been upgraded from
+          0.5.1 to
+          <link xlink:href="https://github.com/matrix-org/dendrite/releases/tag/v0.6.5">0.6.5</link>.
+          Instances configured with split sqlite databases, which has
+          been the default in NixOS, require merging of the federation
+          sender and signing key databases. See upstream
+          <link xlink:href="https://github.com/matrix-org/dendrite/releases/tag/v0.6.0">release
+          notes</link> on version 0.6.0 for details on database changes.
+        </para>
+      </listitem>
       <listitem>
         <para>
           The existing <literal>pkgs.opentelemetry-collector</literal>
diff --git a/nixos/doc/manual/release-notes/rl-2205.section.md b/nixos/doc/manual/release-notes/rl-2205.section.md
index f56bc3d12872..b9402ba5f9dd 100644
--- a/nixos/doc/manual/release-notes/rl-2205.section.md
+++ b/nixos/doc/manual/release-notes/rl-2205.section.md
@@ -293,6 +293,14 @@ In addition to numerous new and upgraded packages, this release has the followin
 
   This breaks back-compat so it's not possible to mix-and-match with previous versions of nixpkgs. In exchange, it now becomes possible to use the providers from [nixpkgs-terraform-providers-bin](https://github.com/numtide/nixpkgs-terraform-providers-bin) directly.
 
+- The `dendrite` package has been upgraded from 0.5.1 to
+  [0.6.5](https://github.com/matrix-org/dendrite/releases/tag/v0.6.5). Instances
+  configured with split sqlite databases, which has been the default
+  in NixOS, require merging of the federation sender and signing key
+  databases. See upstream [release
+  notes](https://github.com/matrix-org/dendrite/releases/tag/v0.6.0)
+  on version 0.6.0 for details on database changes.
+
 - The existing `pkgs.opentelemetry-collector` has been moved to
   `pkgs.opentelemetry-collector-contrib` to match the actual source being the
   "contrib" edition. `pkgs.opentelemetry-collector` is now the actual core
diff --git a/nixos/modules/services/misc/dendrite.nix b/nixos/modules/services/misc/dendrite.nix
index c967fc3a362a..b2885b094153 100644
--- a/nixos/modules/services/misc/dendrite.nix
+++ b/nixos/modules/services/misc/dendrite.nix
@@ -110,6 +110,15 @@ in
             '';
           };
         };
+        options.app_service_api.database = {
+          connection_string = lib.mkOption {
+            type = lib.types.str;
+            default = "file:federationapi.db";
+            description = ''
+              Database for the Appservice API.
+            '';
+          };
+        };
         options.client_api = {
           registration_disabled = lib.mkOption {
             type = lib.types.bool;
@@ -120,6 +129,91 @@ in
             '';
           };
         };
+        options.federation_api.database = {
+          connection_string = lib.mkOption {
+            type = lib.types.str;
+            default = "file:federationapi.db";
+            description = ''
+              Database for the Federation API.
+            '';
+          };
+        };
+        options.key_server.database = {
+          connection_string = lib.mkOption {
+            type = lib.types.str;
+            default = "file:keyserver.db";
+            description = ''
+              Database for the Key Server (for end-to-end encryption).
+            '';
+          };
+        };
+        options.media_api = {
+          database = {
+            connection_string = lib.mkOption {
+              type = lib.types.str;
+              default = "file:mediaapi.db";
+              description = ''
+                Database for the Media API.
+              '';
+            };
+          };
+          base_path = lib.mkOption {
+            type = lib.types.str;
+            default = "${workingDir}/media_store";
+            description = ''
+              Storage path for uploaded media.
+            '';
+          };
+        };
+        options.room_server.database = {
+          connection_string = lib.mkOption {
+            type = lib.types.str;
+            default = "file:roomserver.db";
+            description = ''
+              Database for the Room Server.
+            '';
+          };
+        };
+        options.sync_api.database = {
+          connection_string = lib.mkOption {
+            type = lib.types.str;
+            default = "file:syncserver.db";
+            description = ''
+              Database for the Sync API.
+            '';
+          };
+        };
+        options.user_api = {
+          account_database = {
+            connection_string = lib.mkOption {
+              type = lib.types.str;
+              default = "file:userapi_accounts.db";
+              description = ''
+                Database for the User API, accounts.
+              '';
+            };
+          };
+          device_database = {
+            connection_string = lib.mkOption {
+              type = lib.types.str;
+              default = "file:userapi_devices.db";
+              description = ''
+                Database for the User API, devices.
+              '';
+            };
+          };
+        };
+        options.mscs = {
+          database = {
+            connection_string = lib.mkOption {
+              type = lib.types.str;
+              default = "file:mscs.db";
+              description = ''
+                Database for exerimental MSC's.
+              '';
+            };
+          };
+        };
       };
       default = { };
       description = ''
diff --git a/pkgs/servers/dendrite/default.nix b/pkgs/servers/dendrite/default.nix
index 3d92b2cd4bac..b1c0f1424d9f 100644
--- a/pkgs/servers/dendrite/default.nix
+++ b/pkgs/servers/dendrite/default.nix
@@ -2,16 +2,16 @@
 
 buildGoModule rec {
   pname = "matrix-dendrite";
-  version = "0.5.1";
+  version = "0.6.5";
 
   src = fetchFromGitHub {
     owner = "matrix-org";
     repo = "dendrite";
     rev = "v${version}";
-    sha256 = "1HCVWSxXOR2syN+dLDSvrNzYHTj/vXZRHkXhU0f3m1k=";
+    sha256 = "jSn2awZsfsniSOTNkaEdQw/sZm7nUfiMntsxigy/51Y=";
   };
 
-  vendorSha256 = "sha256-RqEt0RAsKWKy6NvMzulqY56nZ7fIxgJkgN/WpEZ3F2I=";
+  vendorSha256 = "sha256-B4d3FGXy8TrED3oikTjETQso/AtEfIWWcdY6FykD/8A=";
 
   passthru.tests = {
     inherit (nixosTests) dendrite;