diff --git a/pkgs/servers/monitoring/zabbix/versions.nix b/pkgs/servers/monitoring/zabbix/versions.nix
index 8b6a6bdc7c03..2b00d8939412 100644
--- a/pkgs/servers/monitoring/zabbix/versions.nix
+++ b/pkgs/servers/monitoring/zabbix/versions.nix
@@ -9,11 +9,6 @@ generic: {
     hash = "sha256-r9VNy3bRuFvJAL+ZQEbKjCAS3+AvetnWAwFjLxFVuGU=";
     vendorHash = null;
   };
-  v64 = generic {
-    version = "6.4.20";
-    hash = "sha256-tFsg2Jq8Uaa5YULGUu1kXLkxyJuA3YGeSfJ4DPfOHkk=";
-    vendorHash = null;
-  };
   v60 = generic {
     version = "6.0.36";
     hash = "sha256-Ne0OY6NGzTYOn3sDVd+5tfawBu5VBjxNRtlxasubGCk=";
diff --git a/pkgs/top-level/aliases.nix b/pkgs/top-level/aliases.nix
index 8fbe6fcfda83..57291469d208 100644
--- a/pkgs/top-level/aliases.nix
+++ b/pkgs/top-level/aliases.nix
@@ -1929,6 +1929,7 @@ mapAliases {
   ### Z ###
 
   zabbix50 = throw "'zabbix50' has been removed, it would have reached its End of Life a few days after the release of NixOS 25.05. Consider upgrading to 'zabbix60' or 'zabbix70'.";
+  zabbix64 = throw "'zabbix64' has been removed because it reached its End of Life. Consider upgrading to 'zabbix70'.";
   zeroadPackages = recurseIntoAttrs {
     zeroad = lib.warnOnInstantiate "'zeroadPackages.zeroad' has been renamed to 'zeroad'" zeroad; # Added 2025-03-22
     zeroad-data = lib.warnOnInstantiate "'zeroadPackages.zeroad-data' has been renamed to 'zeroad-data'" zeroad-data; # Added 2025-03-22
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index 516952a47bb4..df0d13442b07 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -12577,7 +12577,6 @@ with pkgs;
   zabbix72 = recurseIntoAttrs (zabbixFor "v72");
   zabbix70 = recurseIntoAttrs (zabbixFor "v70");
   zabbix60 = recurseIntoAttrs (zabbixFor "v60");
-  zabbix64 = recurseIntoAttrs (zabbixFor "v64");
 
   zabbix = zabbix60;