diff --git a/pkgs/by-name/ap/apriltag/package.nix b/pkgs/by-name/ap/apriltag/package.nix
index 6438f8e3bac8..b8f302fe1d56 100644
--- a/pkgs/by-name/ap/apriltag/package.nix
+++ b/pkgs/by-name/ap/apriltag/package.nix
@@ -9,7 +9,6 @@
 
 let
   opencv4WithGtk = python3Packages.opencv4.override {
-    enableGtk2 = true; # For GTK2 support
     enableGtk3 = true; # For GTK3 support
   };
 in
diff --git a/pkgs/development/libraries/opencv/4.x.nix b/pkgs/development/libraries/opencv/4.x.nix
index 85163117298f..1c4a1b08f1fd 100644
--- a/pkgs/development/libraries/opencv/4.x.nix
+++ b/pkgs/development/libraries/opencv/4.x.nix
@@ -54,8 +54,6 @@
   enableIpp ? false,
   enablePython ? false,
   pythonPackages,
-  enableGtk2 ? false,
-  gtk2,
   enableGtk3 ? false,
   gtk3,
   enableVtk ? false,
@@ -360,9 +358,6 @@ effectiveStdenv.mkDerivation {
   ++ optionals (effectiveStdenv.buildPlatform == effectiveStdenv.hostPlatform) [
     hdf5
   ]
-  ++ optionals enableGtk2 [
-    gtk2
-  ]
   ++ optionals enableGtk3 [
     gtk3
   ]
@@ -649,7 +644,6 @@ effectiveStdenv.mkDerivation {
       opencv4-tests = callPackage ./tests.nix {
         inherit
           enableGStreamer
-          enableGtk2
           enableGtk3
           runAccuracyTests
           runPerformanceTests
diff --git a/pkgs/development/libraries/opencv/tests.nix b/pkgs/development/libraries/opencv/tests.nix
index 09e2d08f97c5..fe9f73c42e83 100644
--- a/pkgs/development/libraries/opencv/tests.nix
+++ b/pkgs/development/libraries/opencv/tests.nix
@@ -1,6 +1,5 @@
 {
   enableGStreamer,
-  enableGtk2,
   enableGtk3,
   gst_all_1,
   lib,
@@ -76,7 +75,7 @@ runCommand "opencv4-tests"
       #"dnn" #- some caffe tests failed, probably because github workflow also downloads additional models
     ]
     ++ optionals (!isAarch64 && enableGStreamer) [ "gapi" ]
-    ++ optionals (enableGtk2 || enableGtk3) [ "highgui" ];
+    ++ optionals enableGtk3 [ "highgui" ];
 
     inherit runPerformanceTests;
 
diff --git a/pkgs/development/r-modules/default.nix b/pkgs/development/r-modules/default.nix
index 78cfcb1a8470..182fb61463b8 100644
--- a/pkgs/development/r-modules/default.nix
+++ b/pkgs/development/r-modules/default.nix
@@ -3140,7 +3140,7 @@ let
     opencv =
       let
         opencvGtk = pkgs.opencv.override (old: {
-          enableGtk2 = true;
+          enableGtk3 = true;
         });
       in
       old.opencv.overrideAttrs (attrs: {
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index ca4b995a58d2..141bd30f880c 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -12235,7 +12235,6 @@ self: super: with self; {
       enableLto = !stdenv.hostPlatform.isLinux; # https://github.com/NixOS/nixpkgs/issues/343123
       enableUnfree = false; # prevents cache
       enableIpp = true;
-      enableGtk2 = true;
       enableGtk3 = true;
       enableVtk = true;
       enableFfmpeg = true;