diff --git a/pkgs/development/python-modules/nplusone/default.nix b/pkgs/development/python-modules/nplusone/default.nix
index d9a340d82491..898d209d9138 100644
--- a/pkgs/development/python-modules/nplusone/default.nix
+++ b/pkgs/development/python-modules/nplusone/default.nix
@@ -8,7 +8,6 @@
 , mock
 , peewee
 , pytest-django
-, pytest-pythonpath
 , pytestCheckHook
 , six
 , sqlalchemy
@@ -38,7 +37,6 @@ buildPythonPackage rec {
     mock
     peewee
     pytest-django
-    pytest-pythonpath
     pytestCheckHook
     sqlalchemy
     webtest
@@ -54,6 +52,7 @@ buildPythonPackage rec {
 
   postPatch = ''
     substituteInPlace pytest.ini \
+      --replace "python_paths" "pythonpath" \
       --replace "--cov nplusone --cov-report term-missing" ""
   '';
 
diff --git a/pkgs/development/python-modules/pytest-pythonpath/default.nix b/pkgs/development/python-modules/pytest-pythonpath/default.nix
deleted file mode 100644
index 8c3fb48b430e..000000000000
--- a/pkgs/development/python-modules/pytest-pythonpath/default.nix
+++ /dev/null
@@ -1,26 +0,0 @@
-{ buildPythonPackage, fetchPypi, lib, pytest }:
-
-buildPythonPackage rec {
-  pname = "pytest-pythonpath";
-  version = "0.7.4";
-
-  src = fetchPypi {
-    inherit pname version;
-    sha256 = "sha256-ZOGVsjqPjAxjH7Fogtmtb6QTftHylh3dFdUgZc1DXbY=";
-  };
-
-  buildInputs = [ pytest ];
-  checkInputs = [ pytest ];
-
-  checkPhase = ''
-    pytest
-  '';
-
-  meta = with lib; {
-    description =
-      "Pytest plugin for adding to the PYTHONPATH from command line or configs";
-    homepage = "https://github.com/bigsassy/pytest-pythonpath";
-    maintainers = with maintainers; [ cript0nauta ];
-    license = licenses.mit;
-  };
-}
diff --git a/pkgs/development/python-modules/pyverilog/default.nix b/pkgs/development/python-modules/pyverilog/default.nix
index 8e41d26921fa..115014a25bbb 100644
--- a/pkgs/development/python-modules/pyverilog/default.nix
+++ b/pkgs/development/python-modules/pyverilog/default.nix
@@ -5,7 +5,6 @@
 , jinja2
 , ply
 , verilog
-, pytest-pythonpath
 , pytestCheckHook
 }:
 
@@ -32,8 +31,12 @@ buildPythonPackage rec {
     verilog
   ];
 
+  preCheck = ''
+    substituteInPlace pytest.ini \
+      --replace "python_paths" "pythonpath"
+  '';
+
   checkInputs = [
-    pytest-pythonpath
     pytestCheckHook
   ];
 
diff --git a/pkgs/top-level/python-aliases.nix b/pkgs/top-level/python-aliases.nix
index b8ea307917db..05ebce6357c6 100644
--- a/pkgs/top-level/python-aliases.nix
+++ b/pkgs/top-level/python-aliases.nix
@@ -92,6 +92,7 @@ mapAliases ({
   pytest_6 = pytest; # added 2022-02-10
   pytestcov = pytest-cov; # added 2021-01-04
   pytest-pep8 = pytestpep8; # added 2021-01-04
+  pytest-pythonpath = throw "pytest-pythonpath is obsolete as of pytest 7.0.0 and has been removed"; # added 2022-03-09
   pytestpep8 = throw "pytestpep8 was removed because it is abandoned and no longer compatible with pytest v6.0"; # added 2020-12-10
   pytestquickcheck = pytest-quickcheck; # added 2021-07-20
   pytestrunner = pytest-runner; # added 2021-01-04
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 5cfc1521a8ab..989f0680881a 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -7891,8 +7891,6 @@ in {
 
   pytest-pylint = callPackage ../development/python-modules/pytest-pylint { };
 
-  pytest-pythonpath = callPackage ../development/python-modules/pytest-pythonpath { };
-
   pytest-qt = callPackage ../development/python-modules/pytest-qt { };
 
   pytest-quickcheck = callPackage ../development/python-modules/pytest-quickcheck { };