diff --git a/pkgs/development/python-modules/mfusepy/default.nix b/pkgs/development/python-modules/mfusepy/default.nix
new file mode 100644
index 000000000000..668ec2506ec3
--- /dev/null
+++ b/pkgs/development/python-modules/mfusepy/default.nix
@@ -0,0 +1,42 @@
+{
+  lib,
+  stdenv,
+  buildPythonPackage,
+  fetchFromGitHub,
+  setuptools,
+  fuse3,
+}:
+
+let
+  version = "3.0.0";
+in
+buildPythonPackage {
+  pname = "mfusepy";
+  inherit version;
+  pyproject = true;
+
+  src = fetchFromGitHub {
+    owner = "mxmlnkn";
+    repo = "mfusepy";
+    tag = "v${version}";
+    hash = "sha256-a0F8zcPfKwkYgbXMrLZZ3+kkMfn+ohqoBFa98yNr8uE=";
+  };
+
+  # If fuse library path cannot be found, use fuse library path in nixpkgs
+  postPatch = lib.optionalString stdenv.hostPlatform.isLinux ''
+    substituteInPlace mfusepy.py \
+      --replace-fail "_libfuse_path = find_library('fuse3')" '_libfuse_path = "${lib.getLib fuse3}/lib/libfuse3.so.4"'
+  '';
+
+  build-system = [ setuptools ];
+
+  # https://github.com/NixOS/nixpkgs/blob/1e1947e8b7962c914b725e8b821e311229e632ae/doc/packages/fuse.section.md?plain=1#L10
+  pythonImportsCheck = lib.optionals stdenv.hostPlatform.isLinux [ "mfusepy" ];
+
+  meta = {
+    description = "Ctypes bindings for the high-level API in libfuse 2 and 3";
+    homepage = "https://github.com/mxmlnkn/mfusepy";
+    license = lib.licenses.isc;
+    maintainers = with lib.maintainers; [ kyehn ];
+  };
+}
diff --git a/pkgs/development/python-modules/ratarmount/default.nix b/pkgs/development/python-modules/ratarmount/default.nix
index cb4d43ba8153..a49c146a0bf4 100644
--- a/pkgs/development/python-modules/ratarmount/default.nix
+++ b/pkgs/development/python-modules/ratarmount/default.nix
@@ -2,10 +2,10 @@
   lib,
   buildPythonPackage,
   fetchPypi,
-  fusepy,
   indexed-gzip,
   indexed-zstd,
   libarchive-c,
+  mfusepy,
   python-xz,
   pythonOlder,
   rapidgzip,
@@ -16,14 +16,14 @@
 
 buildPythonPackage rec {
   pname = "ratarmount";
-  version = "1.1.2";
+  version = "1.2.1";
   pyproject = true;
 
   disabled = pythonOlder "3.6";
 
   src = fetchPypi {
     inherit pname version;
-    hash = "sha256-XiwtmZ7HGZwjJJrUD3TOP3o19RBwB/Yu09xdwK13+hk=";
+    hash = "sha256-KL4vG5R3uk0NjXXdvCRo/JBpcNNvSUC9ky0aUYGOBqA=";
   };
 
   pythonRelaxDeps = [ "python-xz" ];
@@ -31,10 +31,10 @@ buildPythonPackage rec {
   build-system = [ setuptools ];
 
   dependencies = [
-    fusepy
     indexed-gzip
     indexed-zstd
     libarchive-c
+    mfusepy
     python-xz
     rapidgzip
     rarfile
diff --git a/pkgs/development/python-modules/ratarmountcore/default.nix b/pkgs/development/python-modules/ratarmountcore/default.nix
index 9371d816b4da..1b89f9f8c875 100644
--- a/pkgs/development/python-modules/ratarmountcore/default.nix
+++ b/pkgs/development/python-modules/ratarmountcore/default.nix
@@ -19,7 +19,7 @@
 
 buildPythonPackage rec {
   pname = "ratarmountcore";
-  version = "1.1.2";
+  version = "0.10.1";
   pyproject = true;
 
   disabled = pythonOlder "3.10";
@@ -27,13 +27,19 @@ buildPythonPackage rec {
   src = fetchFromGitHub {
     owner = "mxmlnkn";
     repo = "ratarmount";
-    tag = "v${version}";
-    hash = "sha256-8DjmYYTb0BR5KvtSeI2s7VtYdbRSI+QCjhZfDwqnk3M=";
+    tag = "core-v${version}";
+    hash = "sha256-7xknOpJIjXMr7Z7JD3Jn3oma63hbEZcj/1zQ6FAp5aA=";
     fetchSubmodules = true;
   };
 
   sourceRoot = "${src.name}/core";
 
+  postPatch = ''
+    substituteInPlace tests/test_AutoMountLayer.py \
+      --replace-fail 'f"tests/{name}.tgz.tgz.gz"' 'os.path.join(os.path.dirname(__file__), f"../../tests/{name}.tgz.tgz.gz")' \
+      --replace-fail 'copy_test_file("tests/double-compressed-nested-tar.tgz.tgz")' 'copy_test_file(os.path.join(os.path.dirname(__file__), "../../tests/double-compressed-nested-tar.tgz.tgz"))'
+  '';
+
   build-system = [ setuptools ];
 
   optional-dependencies = {
@@ -85,7 +91,7 @@ buildPythonPackage rec {
   meta = {
     description = "Library for accessing archives by way of indexing";
     homepage = "https://github.com/mxmlnkn/ratarmount/tree/master/core";
-    changelog = "https://github.com/mxmlnkn/ratarmount/blob/core-${src.tag}/core/CHANGELOG.md";
+    changelog = "https://github.com/mxmlnkn/ratarmount/blob/${src.rev}/core/CHANGELOG.md";
     license = lib.licenses.mit;
     maintainers = with lib.maintainers; [ mxmlnkn ];
   };
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index d5b47cbd5970..bf8cf0483ce0 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -9532,6 +9532,8 @@ self: super: with self; {
 
   mficlient = callPackage ../development/python-modules/mficlient { };
 
+  mfusepy = callPackage ../development/python-modules/mfusepy { };
+
   mhcflurry = callPackage ../development/python-modules/mhcflurry { };
 
   mhcgnomes = callPackage ../development/python-modules/mhcgnomes { };