diff --git a/pkgs/development/python-modules/pytest-pudb/default.nix b/pkgs/development/python-modules/pytest-pudb/default.nix
new file mode 100644
index 000000000000..cc3e0db8570f
--- /dev/null
+++ b/pkgs/development/python-modules/pytest-pudb/default.nix
@@ -0,0 +1,36 @@
+{ lib
+, buildPythonPackage
+, fetchFromGitHub
+, pytestCheckHook
+, pytest
+, pudb
+}:
+
+buildPythonPackage rec {
+  pname = "pytest-pudb";
+  version = "0.7.0";
+  format = "setuptools";
+
+  src = fetchFromGitHub {
+    owner = "wronglink";
+    repo = "pytest-pudb";
+    # Repo missing tags for releases https://github.com/wronglink/pytest-pudb/issues/24
+    rev = "a6b3d2f4d35e558d72bccff472ecde9c9d9c69e5";
+    hash = "sha256-gI9p6sXCQaQjWBXaHJCFli6lBh8+pr+KPhz50fv1F7A=";
+  };
+
+  buildInputs = [ pytest ];
+
+  propagatedBuildInputs = [ pudb ];
+
+  nativeCheckInputs = [ pytestCheckHook ];
+
+  pythonImportsCheck = [ "pytest_pudb" ];
+
+  meta = with lib; {
+    description = "Pytest PuDB debugger integration";
+    homepage = "https://github.com/wronglink/pytest-pudb";
+    license = licenses.mit;
+    maintainers = with maintainers; [ thornycrackers ];
+  };
+}
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 84ede43c7a10..21461fc75255 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -7945,6 +7945,8 @@ self: super: with self; {
 
   pytest-mockito = callPackage ../development/python-modules/pytest-mockito { };
 
+  pytest-pudb = callPackage ../development/python-modules/pytest-pudb { };
+
   python-codon-tables = callPackage ../development/python-modules/python-codon-tables { };
 
   python-creole = callPackage ../development/python-modules/python-creole { };