diff --git a/pkgs/applications/science/molecular-dynamics/gromacs/default.nix b/pkgs/applications/science/molecular-dynamics/gromacs/default.nix
index 5867656b2cbc..a62e716248e0 100644
--- a/pkgs/applications/science/molecular-dynamics/gromacs/default.nix
+++ b/pkgs/applications/science/molecular-dynamics/gromacs/default.nix
@@ -45,8 +45,8 @@ let
       }
     else
       {
-        version = "2024.5";
-        hash = "sha256-/s8GsYbN25Qs+0LujaXz6yuZk+aswKLxjRSsCwFEJPM=";
+        version = "2025.0";
+        hash = "sha256-onrTWmRilbvsEpq+aE2dA9Hi4L12sNYl6QVXRqrvroI=";
       };
 
 in stdenv.mkDerivation rec {
@@ -58,7 +58,7 @@ in stdenv.mkDerivation rec {
     inherit (source) hash;
   };
 
-  patches = [ ./pkgconfig.patch ];
+  patches = [ (if enablePlumed then ./pkgconfig-2024.patch else ./pkgconfig-2025.patch) ];
 
   postPatch = lib.optionalString enablePlumed ''
     plumed patch -p -e gromacs-${source.version}
diff --git a/pkgs/applications/science/molecular-dynamics/gromacs/pkgconfig.patch b/pkgs/applications/science/molecular-dynamics/gromacs/pkgconfig-2024.patch
similarity index 100%
rename from pkgs/applications/science/molecular-dynamics/gromacs/pkgconfig.patch
rename to pkgs/applications/science/molecular-dynamics/gromacs/pkgconfig-2024.patch
diff --git a/pkgs/applications/science/molecular-dynamics/gromacs/pkgconfig-2025.patch b/pkgs/applications/science/molecular-dynamics/gromacs/pkgconfig-2025.patch
new file mode 100644
index 000000000000..f5f5e117ed24
--- /dev/null
+++ b/pkgs/applications/science/molecular-dynamics/gromacs/pkgconfig-2025.patch
@@ -0,0 +1,36 @@
+diff --git a/src/external/muparser/muparser.pc.in b/src/external/muparser/muparser.pc.in
+index 646787cb53..d26e84de8f 100644
+--- a/src/external/muparser/muparser.pc.in
++++ b/src/external/muparser/muparser.pc.in
+@@ -1,11 +1,9 @@
+-prefix=@CMAKE_INSTALL_PREFIX@
+-exec_prefix=${prefix}
+-libdir=${prefix}/@CMAKE_INSTALL_LIBDIR@
+-includedir=${prefix}/@CMAKE_INSTALL_INCLUDEDIR@
+-
+-Name: @PACKAGE_NAME@
+-Description: Mathematical expressions parser library
+-Version: @MUPARSER_VERSION@
+-Requires:
+-Libs: -L${libdir} -lmuparser
+-Cflags: -I${includedir} @PKG_CONFIG_FLAGS@
++libdir=@CMAKE_INSTALL_FULL_LIBDIR@
++includedir=@CMAKE_INSTALL_FULL_INCLUDEDIR@
++
++Name: @PACKAGE_NAME@
++Description: Mathematical expressions parser library
++Version: @MUPARSER_VERSION@
++Requires:
++Libs: -L${libdir} -lmuparser
++Cflags: -I${includedir} @PKG_CONFIG_FLAGS@
+diff --git a/src/gromacs/libgromacs.pc.cmakein b/src/gromacs/libgromacs.pc.cmakein
+index af9b5a6dc0..5f58d549bf 100644
+--- a/src/gromacs/libgromacs.pc.cmakein
++++ b/src/gromacs/libgromacs.pc.cmakein
+@@ -1,5 +1,4 @@
+-prefix=@CMAKE_INSTALL_PREFIX@
+-libdir=${prefix}/@CMAKE_INSTALL_LIBDIR@
++libdir=@CMAKE_INSTALL_FULL_LIBDIR
+ 
+ Name: libgromacs@GMX_LIBS_SUFFIX@
+ Description: Gromacs library