diff --git a/nixos/tests/teleport.nix b/nixos/tests/teleport.nix
index 6b8acb332c43..e1dc146ce2da 100644
--- a/nixos/tests/teleport.nix
+++ b/nixos/tests/teleport.nix
@@ -9,7 +9,6 @@ with import ../lib/testing-python.nix { inherit system pkgs; };
 
 let
   packages = with pkgs; {
-    "16" = teleport_16;
     "17" = teleport_17;
     "18" = teleport_18;
   };
diff --git a/pkgs/by-name/te/teleport_16/package.nix b/pkgs/by-name/te/teleport_16/package.nix
deleted file mode 100644
index 2599a4488b81..000000000000
--- a/pkgs/by-name/te/teleport_16/package.nix
+++ /dev/null
@@ -1,17 +0,0 @@
-{
-  buildTeleport,
-  buildGoModule,
-  wasm-bindgen-cli_0_2_95,
-  withRdpClient ? true,
-  extPatches ? [ ],
-}:
-buildTeleport {
-  version = "16.5.18";
-  hash = "sha256-Dikw4y62V7S62K+8EqltXM4RRYPgE2Ad/kZrSS2TEDo=";
-  vendorHash = "sha256-mcDybNt7Mr0HJW272Ulj1oWlfsH2kEp7rNyeonoIjf8=";
-  pnpmHash = "sha256-Sn3rM6l6pADG7GHenlL3NC0oLc4GFZLTzqDHgzInLbk=";
-  cargoHash = "sha256-04zykCcVTptEPGy35MIWG+tROKFzEepLBmn04mSbt7I=";
-
-  wasm-bindgen-cli = wasm-bindgen-cli_0_2_95;
-  inherit buildGoModule withRdpClient extPatches;
-}
diff --git a/pkgs/top-level/aliases.nix b/pkgs/top-level/aliases.nix
index 930bcec70e97..7a11170c6824 100644
--- a/pkgs/top-level/aliases.nix
+++ b/pkgs/top-level/aliases.nix
@@ -1496,6 +1496,7 @@ mapAliases {
   teamspeak_client = throw "'teamspeak_client' has been renamed to/replaced by 'teamspeak3'"; # Converted to throw 2025-10-27
   tegaki-zinnia-japanese = throw "'tegaki-zinnia-japanese' has been removed due to lack of maintenance"; # Added 2025-09-10
   telepathy-haze = throw "'telepathy-haze' has been removed due to being unmaintained and broken since 2023"; # Added 2025-11-04
+  teleport_16 = throw "teleport 16 has been removed as it is EOL. Please upgrade to Teleport 17 or later"; # Added 2025-11-10
   temporalite = throw "'temporalite' has been removed as it is obsolete and unmaintained, please use 'temporal-cli' instead (with `temporal server start-dev`)"; # Added 2025-06-26
   temurin-bin-23 = throw "Temurin 23 has been removed as it has reached its end of life"; # Added 2025-11-04
   temurin-bin-24 = throw "Temurin 24 has been removed as it has reached its end of life"; # Added 2025-10-04