diff --git a/pkgs/development/interpreters/python/tests.nix b/pkgs/development/interpreters/python/tests.nix
index 4ba1b757c3f6..d2bab1b0c8d3 100644
--- a/pkgs/development/interpreters/python/tests.nix
+++ b/pkgs/development/interpreters/python/tests.nix
@@ -114,6 +114,10 @@ let
       nix-pythonprefix-mypy = callPackage ./tests/test_nix_pythonprefix {
         interpreter = python;
       };
+      # Make sure tkinter is importable. See https://github.com/NixOS/nixpkgs/issues/238990
+      tkinter = callPackage ./tests/test_tkinter {
+        interpreter = python;
+      };
     }
   );
 
diff --git a/pkgs/development/interpreters/python/tests/test_tkinter/default.nix b/pkgs/development/interpreters/python/tests/test_tkinter/default.nix
new file mode 100644
index 000000000000..67e67d2d1e97
--- /dev/null
+++ b/pkgs/development/interpreters/python/tests/test_tkinter/default.nix
@@ -0,0 +1,17 @@
+{ interpreter, writeText, runCommand }:
+
+let
+
+  pythonEnv = interpreter.withPackages(ps: [
+    ps.tkinter
+  ]);
+
+  pythonScript = writeText "myscript.py" ''
+    import tkinter
+    print(tkinter)
+  '';
+
+in runCommand "${interpreter.name}-tkinter-test" {} ''
+  ${pythonEnv}/bin/python ${pythonScript}
+  touch $out
+''