diff --git a/pkgs/development/python-modules/mypy-boto3/default.nix b/pkgs/development/python-modules/mypy-boto3/default.nix
index c3d209b0c06e..ec085e1f08f0 100644
--- a/pkgs/development/python-modules/mypy-boto3/default.nix
+++ b/pkgs/development/python-modules/mypy-boto3/default.nix
@@ -58,6 +58,8 @@ rec {
 
   mypy-boto3-amplifybackend = buildMypyBoto3Package "amplifybackend" "1.28.36" "sha256-tx837SLt7DL1bD/bZl0egzVpClfJKaSY6p82yrcHWRQ=";
 
+  mypy-boto3-amplifyuibuilder = buildMypyBoto3Package "amplifyuibuilder" "1.28.54" "sha256-5Y2AacuMzVHdPntUyDts21bUzttM9t8EdBcwV1MHhyU=";
+
   mypy-boto3-cognito-idp = buildMypyBoto3Package "cognito-idp" "1.28.36" "sha256-pnLO62LZvr4sJsye3gWJROY+xHikSe7dX8erBTRXrPc=";
 
   mypy-boto3-ebs = buildMypyBoto3Package "ebs" "1.28.36" "sha256-w9OLKJAn9UBnA7x+uedhplSV8plZRYlBpviU9Gv1Ny8=";
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 4ab221c5479f..a9bac1325e6f 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -7097,6 +7097,8 @@ self: super: with self; {
 
     mypy-boto3-amplifybackend
 
+    mypy-boto3-amplifyuibuilder
+
     mypy-boto3-cognito-idp
 
     mypy-boto3-ebs