diff --git a/pkgs/development/python-modules/cx-freeze/default.nix b/pkgs/development/python-modules/cx-freeze/default.nix
index 639e648dabf0..0f9f1d7fc3b3 100644
--- a/pkgs/development/python-modules/cx-freeze/default.nix
+++ b/pkgs/development/python-modules/cx-freeze/default.nix
@@ -9,6 +9,7 @@
 
   # dependencies
   filelock,
+  freeze-core,
   packaging,
 
   distutils,
@@ -17,7 +18,10 @@
   dmgbuild,
 
   # tests
-  ensureNewerSourcesForZipFilesHook,
+  anyio,
+  bcrypt,
+
+  python,
   pytest-mock,
   pytestCheckHook,
   versionCheckHook,
@@ -36,15 +40,9 @@ buildPythonPackage rec {
     hash = "sha256-tV2i0o6D/Cz0ePYgJN+c4VgMkhVhO/2xhPX8vsasFPs=";
   };
 
-  patches = [
-    # ValueError: '/nix/store/33ajdw6s479bg0ydhk0zqrxi6p989gbl-python3.12-pytest-8.3.5/lib/python3.12/site-packages'
-    # is not in the subpath of '/nix/store/fqm9bqqlmaqqr02qbalm1bazp810qfiw-python3-3.12.9'
-    ./fix-tests-relative-path.patch
-  ];
-
   postPatch = ''
     substituteInPlace pyproject.toml \
-      --replace-fail "setuptools>=77.0.3,<=80.4.0" "setuptools>=77.0.3"
+      --replace-fail "setuptools>=78.1.1,<81" "setuptools"
   '';
 
   build-system = [
@@ -60,6 +58,7 @@ buildPythonPackage rec {
   dependencies = [
     distutils
     filelock
+    freeze-core
     packaging
     setuptools
   ]
@@ -84,10 +83,16 @@ buildPythonPackage rec {
     writableTmpDirAsHomeHook
     versionCheckHook
   ];
+
   versionCheckProgram = "${placeholder "out"}/bin/cxfreeze";
 
   preCheck = ''
     rm -rf cx_Freeze
+
+    substituteInPlace tests/conftest.py \
+      --replace-fail \
+        "Path(pytest.__file__).parent.parent.relative_to(prefix).as_posix()" \
+        "'python/lib/python${lib.versions.majorMinor python.version}/site-packages/'"
   '';
 
   disabledTests = [
@@ -96,6 +101,7 @@ buildPythonPackage rec {
     "test_bdist_appimage_target_name"
     "test_bdist_appimage_target_name_and_version"
     "test_bdist_appimage_target_name_and_version_none"
+    "test_bdist_appimage_download_runtime"
 
     # Try to install a module: ValueError: ZIP does not support timestamps before 1980
     "test___main__"
@@ -130,6 +136,44 @@ buildPythonPackage rec {
     "test_valid_icon"
     "test_zip_exclude_packages"
     "test_zip_include_packages"
+
+    # Permission denied .lock
+    "test_anyio"
+    "test_av"
+    "test_argon2"
+    "test_bcrypt"
+    "test_crypto"
+    "test_cryptography"
+    "test_multiprocess"
+    "test_matplotlib"
+    "test_pandas"
+    "test_rasterio"
+    "test_shapely"
+    "test_vtk"
+    "test_ortools"
+    "test_pillow"
+    "test_pyarrow"
+    "test_pymupdf"
+    "test_pyproj"
+    "test_pytz"
+    "test_pyzmq"
+    "test_qt"
+    "test_scipy"
+    "test_skimage"
+    "test_sklearn"
+    "test_setuptools"
+    "test_zoneinfo"
+    "test_zoneinfo_and_tzdata"
+    "test_zeroconf"
+    "test_zstd"
+    "test_bdist_appimage_implicit_skip_build"
+    "test_bdist_deb_simple"
+    "test_bdist_deb"
+    "test_bdist_rpm_simple"
+    "test_bdist_rpm"
+    "test_verify_patchelf_older"
+    "test_egg_info"
+    "test_editable_packages"
   ]
   ++ lib.optionals stdenv.hostPlatform.isDarwin [
     # error: Path /nix/store/xzjghvsg4fhr2vv6h4scihsdrgk4i76w-python3-3.12.9/lib/libpython3.12.dylib