diff --git a/pkgs/development/python-modules/pyinstrument/default.nix b/pkgs/development/python-modules/pyinstrument/default.nix
index 38cec05b049a..060094a6cfd7 100644
--- a/pkgs/development/python-modules/pyinstrument/default.nix
+++ b/pkgs/development/python-modules/pyinstrument/default.nix
@@ -9,7 +9,7 @@
 
 buildPythonPackage rec {
   pname = "pyinstrument";
-  version = "4.7.3";
+  version = "5.0.0";
   format = "pyproject";
 
   disabled = pythonOlder "3.7";
@@ -18,7 +18,7 @@ buildPythonPackage rec {
     owner = "joerick";
     repo = pname;
     tag = "v${version}";
-    hash = "sha256-Dvpx6Bf4obHL3inzIHhOrM3u/7X+0NRfEAyynDjtEwE=";
+    hash = "sha256-uJ9KRgSETuxpeEIpBKFz66+Qci86jy36lKkUKpvmKIg=";
   };
 
   nativeBuildInputs = [