diff --git a/pkgs/development/python-modules/dnfile/default.nix b/pkgs/development/python-modules/dnfile/default.nix
new file mode 100644
index 000000000000..83ce068a760f
--- /dev/null
+++ b/pkgs/development/python-modules/dnfile/default.nix
@@ -0,0 +1,48 @@
+{ lib
+, buildPythonPackage
+, fetchFromGitHub
+, pefile
+, pytestCheckHook
+, pythonOlder
+}:
+
+buildPythonPackage rec {
+  pname = "dnfile";
+  version = "0.12.0";
+  format = "setuptools";
+
+  disabled = pythonOlder "3.7";
+
+  src = fetchFromGitHub {
+    owner = "malwarefrank";
+    repo = pname;
+    rev = "refs/tags/v${version}";
+    hash = "sha256-r3DupEyMEXOFeSDo9k0LmGM/TGMbbpVW7zCoUA4oG8Y=";
+    fetchSubmodules = true;
+  };
+
+  postPatch = ''
+    substituteInPlace setup.py \
+      --replace "pytest-runner" ""
+  '';
+
+  propagatedBuildInputs = [
+    pefile
+  ];
+
+  checkInputs = [
+    pytestCheckHook
+  ];
+
+  pythonImportsCheck = [
+    "dnfile"
+  ];
+
+  meta = with lib; {
+    description = "Module to parse .NET executable files";
+    homepage = "hhttps://github.com/malwarefrank/dnfile";
+    changelog = "https://github.com/malwarefrank/dnfile/blob/v${version}/HISTORY.rst";
+    license = with licenses; [ mit ];
+    maintainers = with maintainers; [ fab ];
+  };
+}
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index de076425e89b..d4566b563ff4 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -2678,6 +2678,8 @@ self: super: with self; {
 
   dnachisel = callPackage ../development/python-modules/dnachisel { };
 
+  dnfile = callPackage ../development/python-modules/dnfile { };
+
   dnslib = callPackage ../development/python-modules/dnslib { };
 
   dnspython = callPackage ../development/python-modules/dnspython { };