diff --git a/pkgs/applications/science/biology/last/default.nix b/pkgs/applications/science/biology/last/default.nix
index 3bc84e414bb7..85fb1ca80ac6 100644
--- a/pkgs/applications/science/biology/last/default.nix
+++ b/pkgs/applications/science/biology/last/default.nix
@@ -9,13 +9,13 @@
 
 stdenv.mkDerivation rec {
   pname = "last";
-  version = "1542";
+  version = "1543";
 
   src = fetchFromGitLab {
     owner = "mcfrith";
     repo = "last";
     rev = "refs/tags/${version}";
-    hash = "sha256-ZzvyyecYiBscogfN9/FnDbHg/lqb8y14n9C2KLIqhFA=";
+    hash = "sha256-APHPv7Q64JITfHsvjCThZ6hvGHerk6wjOm32KdTv4k8=";
   };
 
   nativeBuildInputs = [