diff --git a/pkgs/development/python-modules/aspell-python/default.nix b/pkgs/development/python-modules/aspell-python/default.nix
index 7bf0bd859bb6..87784477ccbd 100644
--- a/pkgs/development/python-modules/aspell-python/default.nix
+++ b/pkgs/development/python-modules/aspell-python/default.nix
@@ -6,7 +6,6 @@
   fetchPypi,
   isPy27,
   pytestCheckHook,
-  pythonAtLeast,
   setuptools,
 }:
 
@@ -37,7 +36,7 @@ buildPythonPackage rec {
 
   enabledTestPaths = [ "test/unittests.py" ];
 
-  disabledTests = lib.optionals (pythonAtLeast "3.10") [
+  disabledTests = [
     # https://github.com/WojciechMula/aspell-python/issues/22
     "test_add"
     "test_get"
diff --git a/pkgs/development/python-modules/crashtest/default.nix b/pkgs/development/python-modules/crashtest/default.nix
index 2ecd4adb0975..a2552279172e 100644
--- a/pkgs/development/python-modules/crashtest/default.nix
+++ b/pkgs/development/python-modules/crashtest/default.nix
@@ -2,14 +2,12 @@
   lib,
   buildPythonPackage,
   fetchPypi,
-  pythonAtLeast,
 }:
 
 buildPythonPackage rec {
   pname = "crashtest";
   version = "0.4.1";
   format = "setuptools";
-  disabled = !(pythonAtLeast "3.6");
 
   src = fetchPypi {
     inherit pname version;
diff --git a/pkgs/development/python-modules/csvw/default.nix b/pkgs/development/python-modules/csvw/default.nix
index c5ce235b251e..8bccfd9f3d85 100644
--- a/pkgs/development/python-modules/csvw/default.nix
+++ b/pkgs/development/python-modules/csvw/default.nix
@@ -2,7 +2,6 @@
   lib,
   buildPythonPackage,
   fetchFromGitHub,
-  pythonAtLeast,
   attrs,
   isodate,
   python-dateutil,
@@ -43,8 +42,6 @@ buildPythonPackage rec {
     # this test is flaky on darwin because it depends on the resolution of filesystem mtimes
     # https://github.com/cldf/csvw/blob/45584ad63ff3002a9b3a8073607c1847c5cbac58/tests/test_db.py#L257
     "test_write_file_exists"
-  ]
-  ++ lib.optionals (pythonAtLeast "3.10") [
     # https://github.com/cldf/csvw/issues/58
     "test_roundtrip_escapechar"
     "test_escapequote_escapecharquotechar_final"
diff --git a/pkgs/development/python-modules/edward/default.nix b/pkgs/development/python-modules/edward/default.nix
index 5ca81b8cca4b..2041bc1ce1da 100644
--- a/pkgs/development/python-modules/edward/default.nix
+++ b/pkgs/development/python-modules/edward/default.nix
@@ -2,8 +2,6 @@
   lib,
   buildPythonPackage,
   fetchPypi,
-  isPy27,
-  pythonAtLeast,
   keras,
   numpy,
   scipy,
@@ -16,8 +14,6 @@ buildPythonPackage rec {
   version = "1.3.5";
   format = "setuptools";
 
-  disabled = !(isPy27 || pythonAtLeast "3.4");
-
   src = fetchPypi {
     inherit pname version;
     sha256 = "3818b39e77c26fc1a37767a74fdd5e7d02877d75ed901ead2f40bd03baaa109f";
diff --git a/pkgs/development/python-modules/flake8-future-import/default.nix b/pkgs/development/python-modules/flake8-future-import/default.nix
index 2fd7b83b7ccb..c41bb467c632 100644
--- a/pkgs/development/python-modules/flake8-future-import/default.nix
+++ b/pkgs/development/python-modules/flake8-future-import/default.nix
@@ -2,10 +2,6 @@
   lib,
   buildPythonPackage,
   fetchFromGitHub,
-  isPy27,
-  isPy38,
-  isPy39,
-  pythonAtLeast,
   setuptools,
   flake8,
   six,
@@ -25,15 +21,9 @@ buildPythonPackage rec {
     hash = "sha256-2EcCOx3+PCk9LYpQjHCFNpQVI2Pdi+lWL8R6bNadFe0=";
   };
 
-  patches =
-    lib.optionals (pythonAtLeast "3.10") [ ./fix-annotations-version-11.patch ]
-    ++ lib.optionals (isPy38 || isPy39) [ ./fix-annotations-version-10.patch ]
-    ++ lib.optionals isPy27 [
-      # Upstream disables this test case naturally on python 3, but it also fails
-      # inside NixPkgs for python 2. Since it's going to be deleted, we just skip it
-      # on py2 as well.
-      ./skip-test.patch
-    ];
+  patches = [
+    ./fix-annotations-version-11.patch
+  ];
 
   postPatch = ''
     substituteInPlace "test_flake8_future_import.py" \
diff --git a/pkgs/development/python-modules/flake8-future-import/fix-annotations-version-10.patch b/pkgs/development/python-modules/flake8-future-import/fix-annotations-version-10.patch
deleted file mode 100644
index 2e3062c8ac43..000000000000
--- a/pkgs/development/python-modules/flake8-future-import/fix-annotations-version-10.patch
+++ /dev/null
@@ -1,13 +0,0 @@
-diff --git a/flake8_future_import.py b/flake8_future_import.py
-index 92c3fda..27a1a66 100755
---- a/flake8_future_import.py
-+++ b/flake8_future_import.py
-@@ -76,7 +76,7 @@ UNICODE_LITERALS = Feature(4, 'unicode_literals', (2, 6, 0), (3, 0, 0))
- GENERATOR_STOP = Feature(5, 'generator_stop', (3, 5, 0), (3, 7, 0))
- NESTED_SCOPES = Feature(6, 'nested_scopes', (2, 1, 0), (2, 2, 0))
- GENERATORS = Feature(7, 'generators', (2, 2, 0), (2, 3, 0))
--ANNOTATIONS = Feature(8, 'annotations', (3, 7, 0), (4, 0, 0))
-+ANNOTATIONS = Feature(8, 'annotations', (3, 7, 0), (3, 10, 0))
- 
- 
- # Order important as it defines the error code
diff --git a/pkgs/development/python-modules/flake8-future-import/skip-test.patch b/pkgs/development/python-modules/flake8-future-import/skip-test.patch
deleted file mode 100644
index 300358f9158b..000000000000
--- a/pkgs/development/python-modules/flake8-future-import/skip-test.patch
+++ /dev/null
@@ -1,13 +0,0 @@
-diff --git a/test_flake8_future_import.py b/test_flake8_future_import.py
-index 84fde59..345f23f 100644
---- a/test_flake8_future_import.py
-+++ b/test_flake8_future_import.py
-@@ -230,7 +230,7 @@ class TestBadSyntax(TestCaseBase):
-     """Test using various bad syntax examples from Python's library."""
- 
- 
--@unittest.skipIf(sys.version_info[:2] >= (3, 7), 'flake8 supports up to 3.6')
-+@unittest.skip("Has issue with installed path for flake8 in python2")
- class Flake8TestCase(TestCaseBase):
- 
-     """
diff --git a/pkgs/development/python-modules/httplib2/default.nix b/pkgs/development/python-modules/httplib2/default.nix
index ef7141fbc59d..780fff3ba1a9 100644
--- a/pkgs/development/python-modules/httplib2/default.nix
+++ b/pkgs/development/python-modules/httplib2/default.nix
@@ -11,7 +11,6 @@
   pytest-randomly,
   pytest-timeout,
   pytestCheckHook,
-  pythonAtLeast,
   six,
 }:
 
@@ -42,9 +41,6 @@ buildPythonPackage rec {
 
   __darwinAllowLocalNetworking = true;
 
-  # Don't run tests for older Pythons
-  doCheck = pythonAtLeast "3.9";
-
   disabledTests = [
     # ValueError: Unable to load PEM file.
     # https://github.com/httplib2/httplib2/issues/192#issuecomment-993165140
diff --git a/pkgs/development/python-modules/marshmallow-dataclass/default.nix b/pkgs/development/python-modules/marshmallow-dataclass/default.nix
index 8a725de16474..1638fb33e17a 100644
--- a/pkgs/development/python-modules/marshmallow-dataclass/default.nix
+++ b/pkgs/development/python-modules/marshmallow-dataclass/default.nix
@@ -4,7 +4,6 @@
   fetchFromGitHub,
   marshmallow,
   pytestCheckHook,
-  pythonAtLeast,
   setuptools,
   typeguard,
   typing-inspect,
@@ -39,7 +38,7 @@ buildPythonPackage rec {
     "-Wignore::DeprecationWarning"
   ];
 
-  disabledTests = lib.optionals (pythonAtLeast "3.10") [
+  disabledTests = [
     # TypeError: UserId is not a dataclass and cannot be turned into one.
     "test_newtype"
   ];
diff --git a/pkgs/development/python-modules/persim/default.nix b/pkgs/development/python-modules/persim/default.nix
index ec3669359be4..9c8dbff7ac49 100644
--- a/pkgs/development/python-modules/persim/default.nix
+++ b/pkgs/development/python-modules/persim/default.nix
@@ -10,7 +10,6 @@
   scikit-learn,
   scipy,
   pytestCheckHook,
-  pythonAtLeast,
 }:
 
 buildPythonPackage rec {
@@ -44,7 +43,7 @@ buildPythonPackage rec {
 
   pythonImportsCheck = [ "persim" ];
 
-  disabledTests = lib.optionals (pythonAtLeast "3.10") [
+  disabledTests = [
     # AttributeError: module 'collections' has no attribute 'Iterable'
     "test_empyt_diagram_list"
     "test_empty_diagram_list"
diff --git a/pkgs/development/python-modules/pypass/default.nix b/pkgs/development/python-modules/pypass/default.nix
index ad8e17fc8bee..4e952df17c52 100644
--- a/pkgs/development/python-modules/pypass/default.nix
+++ b/pkgs/development/python-modules/pypass/default.nix
@@ -10,7 +10,6 @@
   gnupg,
   pbr,
   pexpect,
-  pythonAtLeast,
   pytestCheckHook,
   setuptools,
   replaceVars,
@@ -41,8 +40,7 @@ buildPythonPackage rec {
     })
   ];
 
-  # Remove enum34 requirement if Python >= 3.4
-  pythonRemoveDeps = lib.optionals (pythonAtLeast "3.4") [
+  pythonRemoveDeps = [
     "enum34"
   ];
 
diff --git a/pkgs/development/python-modules/scikit-hep-testdata/default.nix b/pkgs/development/python-modules/scikit-hep-testdata/default.nix
index f3ec89b963ca..af270a36233d 100644
--- a/pkgs/development/python-modules/scikit-hep-testdata/default.nix
+++ b/pkgs/development/python-modules/scikit-hep-testdata/default.nix
@@ -9,8 +9,6 @@
   # dependencies
   pyyaml,
   requests,
-  pythonAtLeast,
-  importlib-resources,
 }:
 
 buildPythonPackage rec {
@@ -30,8 +28,7 @@ buildPythonPackage rec {
   dependencies = [
     pyyaml
     requests
-  ]
-  ++ lib.optionals (!pythonAtLeast "3.9") [ importlib-resources ];
+  ];
 
   SKHEP_DATA = 1; # install the actual root files
 
diff --git a/pkgs/development/python-modules/sgmllib3k/default.nix b/pkgs/development/python-modules/sgmllib3k/default.nix
index 9fa707ea0efc..3d488ca98eb0 100644
--- a/pkgs/development/python-modules/sgmllib3k/default.nix
+++ b/pkgs/development/python-modules/sgmllib3k/default.nix
@@ -4,7 +4,6 @@
   fetchPypi,
   isPy27,
   pytestCheckHook,
-  pythonAtLeast,
 }:
 
 buildPythonPackage rec {
@@ -21,7 +20,7 @@ buildPythonPackage rec {
 
   nativeCheckInputs = [ pytestCheckHook ];
 
-  disabledTests = lib.optionals (pythonAtLeast "3.10") [ "test_declaration_junk_chars" ];
+  disabledTests = [ "test_declaration_junk_chars" ];
 
   doCheck = false;
 
diff --git a/pkgs/development/python-modules/urlpy/default.nix b/pkgs/development/python-modules/urlpy/default.nix
index 7e1fc83509aa..db8bd94e8879 100644
--- a/pkgs/development/python-modules/urlpy/default.nix
+++ b/pkgs/development/python-modules/urlpy/default.nix
@@ -4,7 +4,6 @@
   buildPythonPackage,
   publicsuffix2,
   pytestCheckHook,
-  pythonAtLeast,
 }:
 buildPythonPackage rec {
   pname = "urlpy";
@@ -24,7 +23,7 @@ buildPythonPackage rec {
 
   nativeCheckInputs = [ pytestCheckHook ];
 
-  disabledTests = lib.optionals (pythonAtLeast "3.9") [
+  disabledTests = [
     # Fails with "AssertionError: assert 'unknown' == ''"
     "test_unknown_protocol"
   ];
diff --git a/pkgs/development/python-modules/xkcdpass/default.nix b/pkgs/development/python-modules/xkcdpass/default.nix
index 540cc0ebad58..95b2442a3f3c 100644
--- a/pkgs/development/python-modules/xkcdpass/default.nix
+++ b/pkgs/development/python-modules/xkcdpass/default.nix
@@ -4,7 +4,6 @@
   fetchPypi,
   installShellFiles,
   pytestCheckHook,
-  pythonAtLeast,
   setuptools,
 }:
 
@@ -26,7 +25,7 @@ buildPythonPackage rec {
 
   pythonImportsCheck = [ "xkcdpass" ];
 
-  disabledTests = lib.optionals (pythonAtLeast "3.10") [
+  disabledTests = [
     # https://github.com/redacted/XKCD-password-generator/issues/138
     "test_entropy_printout_valid_input"
   ];