diff --git a/pkgs/development/python-modules/ngff-zarr/default.nix b/pkgs/development/python-modules/ngff-zarr/default.nix
new file mode 100644
index 000000000000..9f9385019067
--- /dev/null
+++ b/pkgs/development/python-modules/ngff-zarr/default.nix
@@ -0,0 +1,142 @@
+{
+  lib,
+  buildPythonPackage,
+  dask,
+  dask-image,
+  deepdiff,
+  fetchFromGitHub,
+  hatchling,
+  importlib-resources,
+  itk,
+  itkwasm-downsample,
+  itkwasm-image-io,
+  itkwasm,
+  jsonschema,
+  nibabel,
+  imageio,
+  numpy,
+  imagecodecs,
+  platformdirs,
+  pooch,
+  psutil,
+  pytestCheckHook,
+  rich-argparse,
+  rich,
+  tensorstore,
+  tifffile,
+  typing-extensions,
+  writableTmpDirAsHomeHook,
+  zarr,
+}:
+
+buildPythonPackage (finalAttrs: {
+  pname = "ngff-zarr";
+  version = "0.34.0";
+  pyproject = true;
+
+  src = fetchFromGitHub {
+    owner = "fideus-labs";
+    repo = "ngff-zarr";
+    tag = "py-v${finalAttrs.version}";
+    hash = "sha256-5OiC1NHVEX60oHPwTJ0RN2fQnitwPBMNSfvbMcKAgvc=";
+  };
+
+  sourceRoot = "${finalAttrs.src.name}/py/";
+
+  build-system = [ hatchling ];
+
+  dependencies = [
+    dask
+    importlib-resources
+    itkwasm
+    itkwasm-downsample
+    numpy
+    platformdirs
+    psutil
+    rich
+    rich-argparse
+    typing-extensions
+    zarr
+  ]
+  ++ dask.optional-dependencies.array;
+
+  optional-dependencies = {
+    dask-image = [ dask-image ];
+    # itk = [ itk-filtering ];
+    cli = [
+      # itk-filtering
+      # itk-io
+      # liffile
+      dask
+      dask-image
+      imagecodecs
+      imageio
+      itk
+      itkwasm-image-io
+      nibabel
+      tifffile
+    ]
+    ++ dask.optional-dependencies.distributed;
+    tensorstore = [ tensorstore ];
+    validate = [ jsonschema ];
+  };
+
+  nativeCheckInputs = [
+    deepdiff
+    pooch
+    pytestCheckHook
+    writableTmpDirAsHomeHook
+  ]
+  ++ lib.flatten (builtins.attrValues finalAttrs.passthru.optional-dependencies);
+
+  pythonImportsCheck = [ "ngff_zarr" ];
+
+  disabledTestPaths = [
+    # No CLI tests
+    "test/test_cli_input_to_ngff_image.py"
+    "test/test_cli_output.py"
+    "test/test_cli_relative_paths.py"
+    # Attribute errors
+    "test/test_pyramid_integrity.py"
+    "test/test_multiscales_type.py"
+    "test/test_convert_ome_zarr_version.py"
+    "test/test_itk_image_to_ngff_image.py"
+    # Data missing
+    "test/test_hcs.py"
+    "test/test_hcs_simple.py"
+    "test/test_ngff_validation.py"
+    "test/test_nibabel_image_to_ngff_image.py"
+    # Network access
+    "test/test_from_ngff_zarr_tensorstore.py"
+    "test/test_from_ngff_zarr.py"
+    "test/test_large_serialization.py"
+    "test/test_ngff_image_to_itk_image.py"
+    "test/test_omero.py"
+    "test/test_rfc9_ozx.py"
+    "test/test_to_ngff_zarr_dask_image.py"
+    "test/test_to_ngff_zarr_itk.py"
+    "test/test_to_ngff_zarr_itkwasm.py"
+    "test/test_to_ngff_zarr_rfc2_zarr_v3.py"
+    "test/test_to_ngff_zarr_sharding.py"
+    "test/test_to_ngff_zarr_tensorstore.py"
+    "test/test_to_ngff_zarr_v3_compression.py"
+    # Missing dependencies
+    "test/test_lif_to_ngff_image.py"
+  ];
+
+  disabledTests = [
+    # Assertion errors
+    "test_2d_yx"
+    "test_3d_zyx"
+    "test_smaller_dask_graph"
+    "test_tensorstore_compression"
+  ];
+
+  meta = {
+    description = "Open Microscopy Environment (OME) Next Generation File Format (NGFF) Zarr implementation";
+    homepage = "https://github.com/fideus-labs/ngff-zarr";
+    changelog = "https://github.com/fideus-labs/ngff-zarr/releases/tag/${finalAttrs.src.tag}";
+    license = lib.licenses.isc;
+    maintainers = with lib.maintainers; [ fab ];
+  };
+})
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index e58e99dd34b0..c69083a4038b 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -11090,6 +11090,8 @@ self: super: with self; {
 
   nftables = callPackage ../os-specific/linux/nftables/python.nix { inherit (pkgs) nftables; };
 
+  ngff-zarr = callPackage ../development/python-modules/ngff-zarr { };
+
   nglview = callPackage ../development/python-modules/nglview { };
 
   nh3 = callPackage ../development/python-modules/nh3 { };