diff --git a/pkgs/applications/audio/mousai/default.nix b/pkgs/applications/audio/mousai/default.nix
index 9044d201c457..f61b7c5b7b94 100644
--- a/pkgs/applications/audio/mousai/default.nix
+++ b/pkgs/applications/audio/mousai/default.nix
@@ -1,6 +1,7 @@
 { lib
 , python3
 , fetchFromGitHub
+, substituteAll
 , appstream-glib
 , desktop-file-utils
 , gettext
@@ -13,12 +14,13 @@
 , meson
 , ninja
 , pkg-config
+, pulseaudio
 , wrapGAppsHook
 }:
 
 python3.pkgs.buildPythonApplication rec {
   pname = "mousai";
-  version = "0.4.2";
+  version = "0.6.6";
 
   format = "other";
 
@@ -26,9 +28,16 @@ python3.pkgs.buildPythonApplication rec {
     owner = "SeaDve";
     repo = "Mousai";
     rev = "v${version}";
-    sha256 = "sha256-zH++GGFIz3oxkKOYB4zhY6yL3vENEXxtrv8mZZ+41kU=";
+    sha256 = "sha256-nCbFVFg+nVF8BOBfdzQVgdTRXR5UF18PJFC266yTFwg=";
   };
 
+  patches = [
+    (substituteAll {
+      src = ./paths.patch;
+      pactl = "${lib.getBin pulseaudio}/bin/pactl";
+    })
+  ];
+
   postPatch = ''
     patchShebangs build-aux/meson
   '';
@@ -53,6 +62,7 @@ python3.pkgs.buildPythonApplication rec {
     gtk4
     libadwaita
     librsvg
+    pulseaudio
   ];
 
   propagatedBuildInputs = with python3.pkgs; [
diff --git a/pkgs/applications/audio/mousai/paths.patch b/pkgs/applications/audio/mousai/paths.patch
new file mode 100644
index 000000000000..35b328ad0ea3
--- /dev/null
+++ b/pkgs/applications/audio/mousai/paths.patch
@@ -0,0 +1,13 @@
+diff --git a/src/backend/utils.py b/src/backend/utils.py
+index cebc009..0087c09 100644
+--- a/src/backend/utils.py
++++ b/src/backend/utils.py
+@@ -79,7 +79,7 @@ class Utils:
+     @staticmethod
+     def get_default_audio_sources():
+         pactl_output = subprocess.run(
+-            ['/usr/bin/pactl', 'info'],
++            ['@pactl@', 'info'],
+             stdout=subprocess.PIPE,
+             text=True
+         ).stdout.splitlines()
diff --git a/pkgs/development/libraries/libadwaita/default.nix b/pkgs/development/libraries/libadwaita/default.nix
index 405598c69640..5889c6ee3acf 100644
--- a/pkgs/development/libraries/libadwaita/default.nix
+++ b/pkgs/development/libraries/libadwaita/default.nix
@@ -12,13 +12,16 @@
 , vala
 , gobject-introspection
 , fribidi
+, glib
 , gtk4
+, gnome
+, gsettings-desktop-schemas
 , xvfb-run
 }:
 
 stdenv.mkDerivation rec {
   pname = "libadwaita";
-  version = "1.0.0-alpha.2";
+  version = "1.0.0.alpha.3";
 
   outputs = [ "out" "dev" "devdoc" ];
   outputBin = "devdoc"; # demo app
@@ -28,7 +31,7 @@ stdenv.mkDerivation rec {
     owner = "GNOME";
     repo = "libadwaita";
     rev = version;
-    sha256 = "1yvjdzs5ipmr4gi0l4k6dkqhl9b090kpjc3ll8bv1a6i7yfaf53s";
+    sha256 = "sha256-4ED2m8hZMWbu5sFbkH0W6q05+cYCCkx+ubeDqg3W3a0=";
   };
 
   nativeBuildInputs = [
@@ -54,13 +57,31 @@ stdenv.mkDerivation rec {
   ];
 
   checkInputs = [
+    gnome.adwaita-icon-theme
     xvfb-run
   ];
 
   doCheck = true;
 
   checkPhase = ''
-    xvfb-run meson test
+    runHook preCheck
+
+    testEnvironment=(
+      # Disable portal since we cannot run it in tests.
+      ADW_DISABLE_PORTAL=1
+
+      # AdwSettings needs to be initialized from “org.gnome.desktop.interface” GSettings schema when portal is not used for color scheme.
+      # It will not actually be used since the “color-scheme” key will only have been introduced in GNOME 42, falling back to detecting theme name.
+      # See adw_settings_constructed function in https://gitlab.gnome.org/GNOME/libadwaita/commit/60ec69f0a5d49cad8a6d79e4ecefd06dc6e3db12
+      "XDG_DATA_DIRS=${glib.getSchemaPath gsettings-desktop-schemas}/../.."
+
+      # Tests need a cache directory
+      "HOME=$TMPDIR"
+    )
+    env "''${testEnvironment[@]}" xvfb-run \
+      meson test --print-errorlogs
+
+    runHook postCheck
   '';
 
   postInstall = ''