diff --git a/pkgs/build-support/emacs/wrapper.nix b/pkgs/build-support/emacs/wrapper.nix
index ecfcc0cd52c5..e3c67d03dfa1 100644
--- a/pkgs/build-support/emacs/wrapper.nix
+++ b/pkgs/build-support/emacs/wrapper.nix
@@ -200,7 +200,7 @@ runCommand
       substitute ${./wrapper.sh} $out/bin/$progname \
         --subst-var-by bash ${emacs.stdenv.shell} \
         --subst-var-by wrapperSiteLisp "$deps/share/emacs/site-lisp" \
-        --subst-var-by wrapperSiteLispNative "$deps/share/emacs/native-lisp:" \
+        --subst-var-by wrapperSiteLispNative "$deps/share/emacs/native-lisp" \
         --subst-var prog
       chmod +x $out/bin/$progname
     done
@@ -219,7 +219,7 @@ runCommand
       substitute ${./wrapper.sh} $out/Applications/Emacs.app/Contents/MacOS/Emacs \
         --subst-var-by bash ${emacs.stdenv.shell} \
         --subst-var-by wrapperSiteLisp "$deps/share/emacs/site-lisp" \
-        --subst-var-by wrapperSiteLispNative "$deps/share/emacs/native-lisp:" \
+        --subst-var-by wrapperSiteLispNative "$deps/share/emacs/native-lisp" \
         --subst-var-by prog "$emacs/Applications/Emacs.app/Contents/MacOS/Emacs"
       chmod +x $out/Applications/Emacs.app/Contents/MacOS/Emacs
     fi