diff --git a/pkgs/development/python-modules/chai/default.nix b/pkgs/development/python-modules/chai/default.nix
deleted file mode 100644
index 907de8a8cdc9..000000000000
--- a/pkgs/development/python-modules/chai/default.nix
+++ /dev/null
@@ -1,29 +0,0 @@
-{
-  lib,
-  buildPythonPackage,
-  fetchPypi,
-}:
-
-buildPythonPackage rec {
-  pname = "chai";
-  version = "1.1.2";
-  format = "setuptools";
-
-  src = fetchPypi {
-    inherit pname version;
-    sha256 = "ff8d2b6855f660cd23cd5ec79bd10264d39f24f6235773331b48e7fcd637d6cc";
-  };
-
-  postPatch = ''
-    # python 3.12 compatibility
-    substituteInPlace tests/*.py \
-      --replace "assertEquals" "assertEqual" \
-      --replace "assertNotEquals" "assertNotEqual" \
-      --replace "assert_equals" "assert_equal"
-  '';
-
-  meta = {
-    description = "Mocking, stubbing and spying framework for python";
-    license = lib.licenses.bsd3;
-  };
-}
diff --git a/pkgs/top-level/python-aliases.nix b/pkgs/top-level/python-aliases.nix
index 6163988c63f0..1a4d9b53fafa 100644
--- a/pkgs/top-level/python-aliases.nix
+++ b/pkgs/top-level/python-aliases.nix
@@ -130,6 +130,7 @@ mapAliases {
   can = throw "'can' has been renamed to/replaced by 'python-can'"; # Converted to throw 2025-10-29
   casbin = pycasbin; # added 2025-06-12
   cchardet = throw "'cchardet' has been renamed to/replaced by 'faust-cchardet'"; # Converted to throw 2025-10-29
+  chai = throw "'chai' has been removed as it is no longer maintained upstream and the repository was archived"; # Added 2026-06-08
   characteristic = throw "'characteristic' has been removed because it is no longer maintained upstream"; # Added 2026-01-14
   chart-studio = throw "'chart-studio' has been removed as it is no longer maintained upstream"; # Added 2026-03-12
   chromeprint = throw "'chromaprint' was removed as it depended on m2r which was removed."; # Added 2026-05-27
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 89ca0b58fe5f..eb8471ccecc4 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -2691,8 +2691,6 @@ self: super: with self; {
     callPackage ../development/python-modules/chacha20poly1305-reuseable
       { };
 
-  chai = callPackage ../development/python-modules/chai { };
-
   chainmap = callPackage ../development/python-modules/chainmap { };
 
   chainstream = callPackage ../development/python-modules/chainstream { };