diff --git a/pkgs/development/python-modules/mypy-boto3/default.nix b/pkgs/development/python-modules/mypy-boto3/default.nix
index 5da4f5d260d1..6bdcaf27e01a 100644
--- a/pkgs/development/python-modules/mypy-boto3/default.nix
+++ b/pkgs/development/python-modules/mypy-boto3/default.nix
@@ -600,6 +600,8 @@ rec {
 
   mypy-boto3-rolesanywhere = buildMypyBoto3Package "rolesanywhere" "1.28.36" "sha256-YQKGDMmakoxhIsarmBdvOlqno9Qtm5XqOOuBD3cnhsc=";
 
+  mypy-boto3-route53 = buildMypyBoto3Package "route53" "1.28.36" "sha256-x/T2EZtPlnSFmRkRhU+GSKvRQHpXAL3c+JN6kdmFSuY=";
+
   mypy-boto3-s3 = buildMypyBoto3Package "s3" "1.28.55" "sha256-sAiAn0SOdAdQEtT8VLAXbeC09JvDjjneMMoOdk63UFY=";
 
   mypy-boto3-xray = buildMypyBoto3Package "xray" "1.28.47" "sha256-1OiTpbaBm2aAls4A7ZaZBNAM8DTRuQcwNKJDq3lOKMY=";
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index e414c57a75e3..fcb9396fb323 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -7639,6 +7639,8 @@ self: super: with self; {
 
     mypy-boto3-rolesanywhere
 
+    mypy-boto3-route53
+
     mypy-boto3-s3
 
     mypy-boto3-xray