diff --git a/pkgs/applications/editors/emacs/default.nix b/pkgs/applications/editors/emacs/default.nix
index df04ceaf2c75..faf06ca843c7 100644
--- a/pkgs/applications/editors/emacs/default.nix
+++ b/pkgs/applications/editors/emacs/default.nix
@@ -20,10 +20,6 @@ lib.makeScope pkgs.newScope (self:
 
     emacs28 = callPackage (self.sources.emacs28) inheritedArgs;
 
-    emacs28-gtk2 = self.emacs28.override {
-      withGTK2 = true;
-    };
-
     emacs28-gtk3 = self.emacs28.override {
       withGTK3 = true;
     };
diff --git a/pkgs/applications/editors/emacs/make-emacs.nix b/pkgs/applications/editors/emacs/make-emacs.nix
index ced468858760..cbcd022c3d35 100644
--- a/pkgs/applications/editors/emacs/make-emacs.nix
+++ b/pkgs/applications/editors/emacs/make-emacs.nix
@@ -22,7 +22,6 @@
 , gnutls
 , gpm
 , gsettings-desktop-schemas
-, gtk2-x11
 , gtk3
 , gtk3-x11
 , harfbuzz
@@ -69,7 +68,6 @@
 , withAthena ? false
 , withCsrc ? true
 , withDbus ? stdenv.isLinux
-, withGTK2 ? false
 , withGTK3 ? withPgtk && !noGui
 , withGlibNetworking ? withPgtk || withGTK3 || (withX && withXwidgets)
 , withGpm ? stdenv.isLinux
@@ -95,8 +93,7 @@
 # Options
 , siteStart ? ./site-start.el
 , toolkit ? (
-  if withGTK2 then "gtk2"
-  else if withGTK3 then "gtk3"
+  if withGTK3 then "gtk3"
   else if withMotif then "motif"
   else if withAthena then "athena"
   else "lucid")
@@ -117,14 +114,11 @@
 , WebKit
 }:
 
-assert (withGTK2 && !withNS && variant != "macport") -> withX;
 assert (withGTK3 && !withNS && variant != "macport") -> withX || withPgtk;
 
-assert noGui -> !(withX || withGTK2 || withGTK3 || withNS || variant == "macport");
+assert noGui -> !(withX || withGTK3 || withNS || variant == "macport");
 assert withAcl -> stdenv.isLinux;
 assert withAlsaLib -> stdenv.isLinux;
-assert withGTK2 -> !(withGTK3 || withPgtk);
-assert withGTK3 -> !withGTK2 || withPgtk;
 assert withGpm -> stdenv.isLinux;
 assert withNS -> stdenv.isDarwin && !(withX || variant == "macport");
 assert withPgtk -> withGTK3 && !withX;
@@ -148,7 +142,6 @@ mkDerivation (finalAttrs: {
              else if variant == "macport" then "-macport"
              else if withPgtk then "-pgtk"
              else if withGTK3 then "-gtk3"
-             else if withGTK2 then "-gtk2"
              else "");
   inherit version;
 
@@ -241,8 +234,6 @@ mkDerivation (finalAttrs: {
   ] ++ lib.optionals (stdenv.isLinux && withX) [
     libotf
     m17n_lib
-  ] ++ lib.optionals (withX && withGTK2) [
-    gtk2-x11
   ] ++ lib.optionals (withX && withGTK3) [
     gtk3-x11
   ] ++ lib.optionals (withX && withMotif) [
diff --git a/pkgs/top-level/aliases.nix b/pkgs/top-level/aliases.nix
index 18957005dd3c..e50af1a6a24a 100644
--- a/pkgs/top-level/aliases.nix
+++ b/pkgs/top-level/aliases.nix
@@ -414,6 +414,7 @@ mapAliases {
   elixir_ls = elixir-ls; # Added 2023-03-20
 
   # Emacs
+  emacs28-gtk2 = throw "emacs28-gtk2 was removed because GTK2 is EOL; migrate to emacs28{,-gtk3,-nox} or to more recent versions of Emacs."; # Added 2024-09-20
   emacs28NativeComp = emacs28; # Added 2022-06-08
   emacs28Packages = emacs28.pkgs; # Added 2021-10-04
   emacs28WithPackages = emacs28.pkgs.withPackages; # Added 2021-10-04
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index bcc9af48f461..09d644f76c64 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -29195,7 +29195,6 @@ with pkgs;
 
   inherit (recurseIntoAttrs (callPackage ../applications/editors/emacs { }))
     emacs28
-    emacs28-gtk2
     emacs28-gtk3
     emacs28-nox