diff --git a/pkgs/development/python-modules/mypy-boto3/default.nix b/pkgs/development/python-modules/mypy-boto3/default.nix
index caa385ff73c0..ca1756eb271b 100644
--- a/pkgs/development/python-modules/mypy-boto3/default.nix
+++ b/pkgs/development/python-modules/mypy-boto3/default.nix
@@ -593,8 +593,8 @@ in
       "sha256-/LlMFYC7cJWb9C5JIt0dTEPtl2sPsalSq7mYaFSf3c4=";
 
   mypy-boto3-guardduty =
-    buildMypyBoto3Package "guardduty" "1.40.44"
-      "sha256-vOTVkra2RG5DPgcegpDij+6tjWkY01b6zXoSIze8AZ8=";
+    buildMypyBoto3Package "guardduty" "1.40.53"
+      "sha256-gTfRwvZekeaQ8V7QgepE4S+i134YQiJ1UXDTU+otVZ8=";
 
   mypy-boto3-health =
     buildMypyBoto3Package "health" "1.40.0"