diff --git a/pkgs/applications/graphics/dosage/default.nix b/pkgs/applications/graphics/dosage/default.nix
index 1aaa53948619..d04820afad96 100644
--- a/pkgs/applications/graphics/dosage/default.nix
+++ b/pkgs/applications/graphics/dosage/default.nix
@@ -19,8 +19,6 @@ python3Packages.buildPythonApplication rec {
     colorama imagesize lxml requests setuptools six
   ];
 
-  disabled = python3Packages.pythonOlder "3.3";
-
   meta = {
     description = "A comic strip downloader and archiver";
     mainProgram = "dosage";
diff --git a/pkgs/development/python-modules/ago/default.nix b/pkgs/development/python-modules/ago/default.nix
index ad095c441db2..59aaf184a6fc 100644
--- a/pkgs/development/python-modules/ago/default.nix
+++ b/pkgs/development/python-modules/ago/default.nix
@@ -1,11 +1,7 @@
 { lib
 , buildPythonPackage
 , fetchPypi
-
-, pythonOlder
-
 , pytestCheckHook
-
 , setuptools
 }:
 
@@ -14,8 +10,6 @@ buildPythonPackage rec {
   version = "0.0.95";
   pyproject = true;
 
-  disabled = pythonOlder "3.3";
-
   src = fetchPypi {
     inherit pname version;
     hash = "sha256-0gEPXqw99UTsSOwRYQLgaFkaNFsaWA8ylz24pQX8p0Q=";
diff --git a/pkgs/development/python-modules/anyascii/default.nix b/pkgs/development/python-modules/anyascii/default.nix
index d781a552cca1..3e001d5a1874 100644
--- a/pkgs/development/python-modules/anyascii/default.nix
+++ b/pkgs/development/python-modules/anyascii/default.nix
@@ -1,6 +1,5 @@
 { lib
 , buildPythonPackage
-, pythonOlder
 , fetchPypi
 , pytestCheckHook
 }:
@@ -9,7 +8,6 @@ buildPythonPackage rec {
   pname = "anyascii";
   version = "0.3.2";
   format = "setuptools";
-  disabled = pythonOlder "3.3";
 
   src = fetchPypi {
     inherit pname version;
diff --git a/pkgs/development/python-modules/dronecan/default.nix b/pkgs/development/python-modules/dronecan/default.nix
index 49c5dbb51db4..fc051d105bf6 100644
--- a/pkgs/development/python-modules/dronecan/default.nix
+++ b/pkgs/development/python-modules/dronecan/default.nix
@@ -1,7 +1,6 @@
 { lib
 , buildPythonPackage
 , fetchPypi
-, pythonOlder
 , pytestCheckHook
 }:
 
@@ -9,7 +8,6 @@ buildPythonPackage rec {
   pname = "dronecan";
   version = "1.0.26";
   format = "setuptools";
-  disabled = pythonOlder "3.3";
 
   src = fetchPypi {
     inherit pname version;
diff --git a/pkgs/development/python-modules/dugong/default.nix b/pkgs/development/python-modules/dugong/default.nix
index 7cfc29c9ff81..e66763b23d5e 100644
--- a/pkgs/development/python-modules/dugong/default.nix
+++ b/pkgs/development/python-modules/dugong/default.nix
@@ -2,7 +2,6 @@
 , buildPythonPackage
 , fetchFromGitHub
 , pythonOlder
-, pythonAtLeast
 , pytestCheckHook
 }:
 
@@ -10,7 +9,6 @@ buildPythonPackage rec {
   pname = "dugong";
   version = "3.8.1";
   format = "setuptools";
-  disabled = pythonOlder "3.3";
 
   src = fetchFromGitHub {
     owner = "python-dugong";
diff --git a/pkgs/development/python-modules/gpapi/default.nix b/pkgs/development/python-modules/gpapi/default.nix
index 307e8dfb63b8..1ec16d8216aa 100644
--- a/pkgs/development/python-modules/gpapi/default.nix
+++ b/pkgs/development/python-modules/gpapi/default.nix
@@ -2,7 +2,6 @@
 , cryptography
 , fetchPypi
 , lib
-, pythonOlder
 , protobuf
 , pycryptodome
 , requests
@@ -12,7 +11,6 @@ buildPythonPackage rec {
   version = "0.4.4";
   format = "setuptools";
   pname = "gpapi";
-  disabled = pythonOlder "3.3"; # uses shutil.which(), added in 3.3
 
   src = fetchPypi {
     inherit version pname;
diff --git a/pkgs/development/python-modules/humanfriendly/default.nix b/pkgs/development/python-modules/humanfriendly/default.nix
index d8a3fa92e483..2c60b0b294b8 100644
--- a/pkgs/development/python-modules/humanfriendly/default.nix
+++ b/pkgs/development/python-modules/humanfriendly/default.nix
@@ -1,8 +1,6 @@
 { lib
-, pythonOlder
 , buildPythonPackage
 , fetchPypi
-, monotonic
 }:
 
 buildPythonPackage rec {
@@ -15,8 +13,6 @@ buildPythonPackage rec {
     sha256 = "6b0b831ce8f15f7300721aa49829fc4e83921a9a301cc7f606be6686a2288ddc";
   };
 
-  propagatedBuildInputs = lib.optional (pythonOlder "3.3") monotonic;
-
   # humanfriendly tests depends on coloredlogs which itself depends on
   # humanfriendly. This lead to infinite recursion when trying to
   # build this package so we have to disable the test suite :(
diff --git a/pkgs/development/python-modules/hurry-filesize/default.nix b/pkgs/development/python-modules/hurry-filesize/default.nix
index 62bd1f810f9d..7dd168bf9704 100644
--- a/pkgs/development/python-modules/hurry-filesize/default.nix
+++ b/pkgs/development/python-modules/hurry-filesize/default.nix
@@ -1,9 +1,6 @@
 { lib
 , buildPythonPackage
 , fetchPypi
-
-, pythonOlder
-
 , setuptools
 }:
 
@@ -12,8 +9,6 @@ buildPythonPackage rec {
   version = "0.9";
   pyproject = true;
 
-  disabled = pythonOlder "3.3";
-
   src = fetchPypi {
     pname = "hurry.filesize";
     inherit version;
diff --git a/pkgs/development/python-modules/matlink-gpapi/default.nix b/pkgs/development/python-modules/matlink-gpapi/default.nix
index 3c3cda1bf8db..c88638e4391f 100644
--- a/pkgs/development/python-modules/matlink-gpapi/default.nix
+++ b/pkgs/development/python-modules/matlink-gpapi/default.nix
@@ -2,7 +2,6 @@
 , cryptography
 , fetchPypi
 , lib
-, pythonOlder
 , protobuf
 , pycryptodome
 , requests
@@ -12,7 +11,6 @@ buildPythonPackage rec {
   version = "0.4.4.5";
   format = "setuptools";
   pname = "matlink-gpapi";
-  disabled = pythonOlder "3.3"; # uses shutil.which(), added in 3.3
 
   src = fetchPypi {
     inherit version pname;
diff --git a/pkgs/development/python-modules/mock-open/default.nix b/pkgs/development/python-modules/mock-open/default.nix
index c8ed5926f2f7..413c5f832d29 100644
--- a/pkgs/development/python-modules/mock-open/default.nix
+++ b/pkgs/development/python-modules/mock-open/default.nix
@@ -1,4 +1,4 @@
-{ lib, buildPythonPackage, fetchFromGitHub, pythonOlder, mock }:
+{ lib, buildPythonPackage, fetchFromGitHub }:
 
 buildPythonPackage rec {
   pname = "mock-open";
@@ -13,8 +13,6 @@ buildPythonPackage rec {
     sha256 = "0qlz4y8jqxsnmqg03yp9f87rmnjrvmxm5qvm6n1218gm9k5dixbm";
   };
 
-  propagatedBuildInputs = lib.optional (pythonOlder "3.3") mock;
-
   meta = with lib; {
     homepage = "https://github.com/nivbend/mock-open";
     description = "A better mock for file I/O";
diff --git a/pkgs/development/python-modules/pathlib2/default.nix b/pkgs/development/python-modules/pathlib2/default.nix
index 15238a858990..ab1948005fe8 100644
--- a/pkgs/development/python-modules/pathlib2/default.nix
+++ b/pkgs/development/python-modules/pathlib2/default.nix
@@ -5,7 +5,6 @@
 , pythonOlder
 , scandir ? null
 , glibcLocales
-, mock
 , typing
 }:
 
@@ -21,8 +20,7 @@ buildPythonPackage rec {
 
   propagatedBuildInputs = [ six ]
     ++ lib.optionals (pythonOlder "3.5") [ scandir typing ];
-  nativeCheckInputs = [ glibcLocales ]
-    ++ lib.optional (pythonOlder "3.3") mock;
+  nativeCheckInputs = [ glibcLocales ];
 
   preCheck = ''
     export LC_ALL="en_US.UTF-8"
diff --git a/pkgs/development/python-modules/pykrakenapi/default.nix b/pkgs/development/python-modules/pykrakenapi/default.nix
index 832658039531..02d00afd628f 100644
--- a/pkgs/development/python-modules/pykrakenapi/default.nix
+++ b/pkgs/development/python-modules/pykrakenapi/default.nix
@@ -1,6 +1,5 @@
 { lib
 , buildPythonPackage
-, pythonOlder
 , fetchFromGitHub
 , krakenex
 , pandas
@@ -10,8 +9,6 @@ buildPythonPackage rec {
   pname = "pykrakenapi";
   version = "0.3.2";
 
-  disabled = pythonOlder "3.3";
-
   format = "setuptools";
 
   src = fetchFromGitHub {
diff --git a/pkgs/development/python-modules/pysqlcipher3/default.nix b/pkgs/development/python-modules/pysqlcipher3/default.nix
index 1b573628edda..1bfd129cf21e 100644
--- a/pkgs/development/python-modules/pysqlcipher3/default.nix
+++ b/pkgs/development/python-modules/pysqlcipher3/default.nix
@@ -1,12 +1,10 @@
-{ lib, buildPythonPackage, fetchPypi, pythonOlder, sqlcipher }:
+{ lib, buildPythonPackage, fetchPypi, sqlcipher }:
 
 buildPythonPackage rec {
   pname = "pysqlcipher3";
   version = "1.2.0";
   format = "setuptools";
 
-  disabled = pythonOlder "3.3";
-
   src = fetchPypi {
     inherit pname version;
     hash = "sha256-PIAzgSZVlH6/KagJrFEGsrxpvgJ06szva1j0WAyNBsU=";
diff --git a/pkgs/development/python-modules/theano/default.nix b/pkgs/development/python-modules/theano/default.nix
index 78a2a0836e76..ef8b80953390 100644
--- a/pkgs/development/python-modules/theano/default.nix
+++ b/pkgs/development/python-modules/theano/default.nix
@@ -53,7 +53,7 @@ in buildPythonPackage rec {
   version = "1.0.5";
   format = "setuptools";
 
-  disabled = isPyPy || pythonOlder "2.6" || (isPy3k && pythonOlder "3.3");
+  disabled = isPyPy || pythonOlder "2.6";
 
   src = fetchPypi {
     inherit pname version;
diff --git a/pkgs/tools/system/journalwatch/default.nix b/pkgs/tools/system/journalwatch/default.nix
index 8bb9b605a8f4..0959e6166a0d 100644
--- a/pkgs/tools/system/journalwatch/default.nix
+++ b/pkgs/tools/system/journalwatch/default.nix
@@ -1,9 +1,8 @@
-{ lib, buildPythonPackage, fetchFromGitHub, pythonOlder, systemd, pytest }:
+{ lib, buildPythonPackage, fetchFromGitHub, systemd, pytest }:
 
 buildPythonPackage rec {
   pname = "journalwatch";
   version = "1.1.0";
-  disabled = pythonOlder "3.3";
 
   src = fetchFromGitHub {
     owner = "The-Compiler";