diff --git a/pkgs/applications/networking/gns3/server.nix b/pkgs/applications/networking/gns3/server.nix
index 3e4764a14502..c6ba5fe2b93e 100644
--- a/pkgs/applications/networking/gns3/server.nix
+++ b/pkgs/applications/networking/gns3/server.nix
@@ -47,7 +47,6 @@ python3Packages.buildPythonApplication {
     setuptools
     truststore
     yarl
-    zipstream
   ] ++ lib.optionals (pythonOlder "3.9") [
     importlib-resources
   ];
diff --git a/pkgs/development/python-modules/zipstream/default.nix b/pkgs/development/python-modules/zipstream/default.nix
deleted file mode 100644
index b48d65cf576a..000000000000
--- a/pkgs/development/python-modules/zipstream/default.nix
+++ /dev/null
@@ -1,26 +0,0 @@
-{
-  lib,
-  buildPythonPackage,
-  fetchPypi,
-  nose,
-}:
-
-buildPythonPackage rec {
-  pname = "zipstream";
-  version = "1.1.4";
-  format = "setuptools";
-
-  src = fetchPypi {
-    inherit pname version;
-    sha256 = "01im5anqdyggmwkigqcjg0qw2a5bnn84h33mfaqjjd69a28lpwif";
-  };
-
-  nativeCheckInputs = [ nose ];
-
-  meta = {
-    description = "A zip archive generator";
-    homepage = "https://github.com/allanlei/python-zipstream";
-    license = lib.licenses.gpl3Plus;
-    maintainers = with lib.maintainers; [ ];
-  };
-}
diff --git a/pkgs/top-level/python-aliases.nix b/pkgs/top-level/python-aliases.nix
index 47a9b6fe570c..9d18dc422d50 100644
--- a/pkgs/top-level/python-aliases.nix
+++ b/pkgs/top-level/python-aliases.nix
@@ -573,6 +573,7 @@ mapAliases ({
   zc_buildout_nix = throw "zc_buildout_nix was pinned to a version no longer compatible with other modules";
   zc-buildout221 = zc-buildout; # added 2021-07-21
   zc_lockfile = zc-lockfile; # added 2024-01-06
+  zipstream = throw "zipstream has been removed, because it has been unmaintained for 6 years and there are no dependent packages"; # added 2024-05-26
   zipstream-new = throw "zipstream-new has been removed, because it  was packaged as a dependency for octoprint, which has switched to zipstream-ng since."; # added 2024-01-05
   zope_broken = throw "zope_broken has been removed because it is obsolete and not needed in zodb>=3.10"; # added 2023-07-26
   zope_component = zope-component; # added 2023-07-28
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 061b75dfcffe..d20910a5302c 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -17381,8 +17381,6 @@ self: super: with self; {
 
   zipp = callPackage ../development/python-modules/zipp { };
 
-  zipstream = callPackage ../development/python-modules/zipstream { };
-
   zipstream-ng = callPackage ../development/python-modules/zipstream-ng { };
 
   zlib-ng = callPackage ../development/python-modules/zlib-ng {