diff --git a/pkgs/development/python-modules/pyqtgraph/default.nix b/pkgs/development/python-modules/pyqtgraph/default.nix
index dfa33aba6ff7..fcc6e51f8bbf 100644
--- a/pkgs/development/python-modules/pyqtgraph/default.nix
+++ b/pkgs/development/python-modules/pyqtgraph/default.nix
@@ -3,6 +3,7 @@
   stdenv,
   buildPythonPackage,
   fetchFromGitHub,
+  fetchpatch,
 
   # build-system
   setuptools,
@@ -37,6 +38,17 @@ buildPythonPackage rec {
     hash = "sha256-MUwg1v6oH2TGmJ14Hp9i6KYierJbzPggK59QaHSXHVA=";
   };
 
+  patches = [
+    # Fixes a segmentation fault in tests with Qt 6.10. See:
+    # https://github.com/pyqtgraph/pyqtgraph/issues/3390
+    # The patch is the merge commit of:
+    # https://github.com/pyqtgraph/pyqtgraph/pull/3370
+    (fetchpatch {
+      url = "https://github.com/pyqtgraph/pyqtgraph/commit/bf38b8527e778c9c0bb653bc0df7bb36018dcbae.patch";
+      hash = "sha256-Tv4QK/OZvmDO3MOjswjch7DpF96U1uRN0dr8NIQ7+LY=";
+    })
+  ];
+
   build-system = [
     setuptools
   ];
@@ -79,11 +91,6 @@ buildPythonPackage rec {
       "test_rescaleData"
     ];
 
-  disabledTestPaths = [
-    # Segmentation fault
-    "tests/test_qpainterpathprivate.py"
-  ];
-
   meta = {
     description = "Scientific Graphics and GUI Library for Python";
     homepage = "https://www.pyqtgraph.org/";