diff --git a/pkgs/development/python-modules/pytest-notebook/default.nix b/pkgs/development/python-modules/pytest-notebook/default.nix
index 6d24c8c2ac60..5273c7540626 100644
--- a/pkgs/development/python-modules/pytest-notebook/default.nix
+++ b/pkgs/development/python-modules/pytest-notebook/default.nix
@@ -38,6 +38,11 @@ buildPythonPackage rec {
     hash = "sha256-LoK0wb7rAbVbgyURCbSfckWvJDef3tPY+7V4YU1IBRU=";
   };
 
+  patches = [
+    # Rename pytest_collect_file hook parameter `path` -> `file_path` for pytest 9.
+    ./pytest9-collect-hook.patch
+  ];
+
   postPatch = ''
     # we disable the tests relying on coverage for unrelated reasons
     substituteInPlace tests/test_execution.py \
diff --git a/pkgs/development/python-modules/pytest-notebook/pytest9-collect-hook.patch b/pkgs/development/python-modules/pytest-notebook/pytest9-collect-hook.patch
new file mode 100644
index 000000000000..c35a77dd6159
--- /dev/null
+++ b/pkgs/development/python-modules/pytest-notebook/pytest9-collect-hook.patch
@@ -0,0 +1,31 @@
+--- a/pytest_notebook/plugin.py
++++ b/pytest_notebook/plugin.py
+@@ -11,6 +11,7 @@
+
+ """
+ import os
++from fnmatch import fnmatch
+ import shlex
+
+ import pytest
+@@ -263,16 +264,16 @@
+     return NBRegressionFixture(**kwargs)
+
+
+-def pytest_collect_file(path, parent):
++def pytest_collect_file(file_path, parent):
+     """Collect Jupyter notebooks using the specified pytest hook."""
+     kwargs, other_args = gather_config_options(parent.config)
+     if other_args.get("nb_test_files", False) and any(
+-        path.fnmatch(pat) for pat in other_args.get("nb_file_fnmatch", ["*.ipynb"])
++        fnmatch(file_path.name, pat) for pat in other_args.get("nb_file_fnmatch", ["*.ipynb"])
+     ):
+         try:
+-            return JupyterNbCollector.from_parent(parent, fspath=path)
++            return JupyterNbCollector.from_parent(parent, path=file_path)
+         except AttributeError:
+-            return JupyterNbCollector(path, parent)
++            return JupyterNbCollector(file_path, parent)
+
+
+ class JupyterNbCollector(pytest.File):