diff --git a/pkgs/tools/security/nitrokey-app2/default.nix b/pkgs/tools/security/nitrokey-app2/default.nix
index 377d212119f5..cecbb27c6e00 100644
--- a/pkgs/tools/security/nitrokey-app2/default.nix
+++ b/pkgs/tools/security/nitrokey-app2/default.nix
@@ -1,30 +1,38 @@
 {
   lib,
   stdenv,
-  python3,
+  buildPythonApplication,
   fetchFromGitHub,
-  wrapQtAppsHook,
-  qtbase,
-  qtwayland,
-  qtsvg,
+  poetry-core,
+  fido2,
+  nitrokey,
+  pyside6,
+  usb-monitor,
+  qt6,
 }:
 
-python3.pkgs.buildPythonApplication rec {
-  pname = "nitrokey-app2";
-  version = "2.3.5";
-  pyproject = true;
+let
+  inherit (qt6)
+    wrapQtAppsHook
+    qtbase
+    qtwayland
+    qtsvg
+    ;
+in
 
-  disabled = python3.pythonOlder "3.9";
+buildPythonApplication rec {
+  pname = "nitrokey-app2";
+  version = "2.4.1";
+  pyproject = true;
 
   src = fetchFromGitHub {
     owner = "Nitrokey";
     repo = "nitrokey-app2";
     tag = "v${version}";
-    hash = "sha256-zhTDr4GyE4jridK3ee8ae3v5behMbuo86q9WdrBVqQg=";
+    hash = "sha256-nzhhtnKKOHA+Cw1y+BpYsyQklzkDnmFRKGIfaJ/dmaQ=";
   };
 
-  nativeBuildInputs = with python3.pkgs; [
-    poetry-core
+  nativeBuildInputs = [
     wrapQtAppsHook
   ];
 
@@ -36,7 +44,12 @@ python3.pkgs.buildPythonApplication rec {
     qtsvg
   ];
 
-  propagatedBuildInputs = with python3.pkgs; [
+  build-system = [
+    poetry-core
+  ];
+
+  dependencies = [
+    fido2
     nitrokey
     pyside6
     usb-monitor
@@ -53,6 +66,12 @@ python3.pkgs.buildPythonApplication rec {
     install -Dm755 meta/nk-app2.png $out/share/icons/hicolor/128x128/apps/com.nitrokey.nitrokey-app2.png
   '';
 
+  # wrapQtApps only wrapps binary files and normally skips python programs.
+  # Manually pass the qtWrapperArgs from wrapQtAppsHook to wrap python programs.
+  preFixup = ''
+    makeWrapperArgs+=("''${qtWrapperArgs[@]}")
+  '';
+
   meta = with lib; {
     description = "This application allows to manage Nitrokey 3 devices";
     homepage = "https://github.com/Nitrokey/nitrokey-app2";
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index 766a29d55138..193fd3a3b763 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -15397,7 +15397,7 @@ with pkgs;
 
   nitrokey-app = libsForQt5.callPackage ../tools/security/nitrokey-app { };
 
-  nitrokey-app2 = qt6Packages.callPackage ../tools/security/nitrokey-app2 { };
+  nitrokey-app2 = python3Packages.callPackage ../tools/security/nitrokey-app2 { };
 
   hy = with python3Packages; toPythonApplication hy;