diff --git a/pkgs/development/python-modules/itkwasm-image-io-wasi/default.nix b/pkgs/development/python-modules/itkwasm-image-io-wasi/default.nix
new file mode 100644
index 000000000000..8bee064ec41d
--- /dev/null
+++ b/pkgs/development/python-modules/itkwasm-image-io-wasi/default.nix
@@ -0,0 +1,46 @@
+{
+  lib,
+  buildPythonPackage,
+  fetchPypi,
+  hatch-vcs,
+  hatchling,
+  importlib-resources,
+  itkwasm,
+  writableTmpDirAsHomeHook,
+}:
+
+buildPythonPackage (finalAttrs: {
+  pname = "itkwasm-image-io-wasi";
+  version = "1.6.1";
+  pyproject = true;
+
+  src = fetchPypi {
+    pname = "itkwasm_image_io_wasi";
+    inherit (finalAttrs) version;
+    hash = "sha256-g3w/OPU9N1GxJkW9kKrOvGtVPRVb6zTy5n2nB5WU7+Q=";
+  };
+
+  build-system = [
+    hatch-vcs
+    hatchling
+  ];
+
+  nativeBuildInputs = [ writableTmpDirAsHomeHook ];
+
+  dependencies = [
+    importlib-resources
+    itkwasm
+  ];
+
+  pythonImportsCheck = [ "itkwasm_image_io_wasi" ];
+
+  # No tests available
+  doCheck = false;
+
+  meta = {
+    description = "Input and output for scientific and medical image file formats";
+    homepage = "https://pypi.org/project/itkwasm-image-io-wasi";
+    license = lib.licenses.asl20;
+    maintainers = with lib.maintainers; [ fab ];
+  };
+})
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 24de9276aae4..e58e99dd34b0 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -7817,6 +7817,8 @@ self: super: with self; {
     callPackage ../development/python-modules/itkwasm-image-io-emscripten
       { };
 
+  itkwasm-image-io-wasi = callPackage ../development/python-modules/itkwasm-image-io-wasi { };
+
   itsdangerous = callPackage ../development/python-modules/itsdangerous { };
 
   itunespy = callPackage ../development/python-modules/itunespy { };