diff --git a/pkgs/applications/video/streamlink/default.nix b/pkgs/applications/video/streamlink/default.nix
index 1c1a0fb3d84c..4abc2b6546aa 100644
--- a/pkgs/applications/video/streamlink/default.nix
+++ b/pkgs/applications/video/streamlink/default.nix
@@ -2,6 +2,7 @@
 , python3Packages
 , rtmpdump
 , ffmpeg
+, fetchpatch
 }:
 
 python3Packages.buildPythonApplication rec {
@@ -33,11 +34,14 @@ python3Packages.buildPythonApplication rec {
     ffmpeg
   ];
 
-  # note that upstream currently uses requests 2.25.1 in Windows builds
-  postPatch = ''
-    substituteInPlace setup.py \
-      --replace 'requests>=2.26.0,<3.0' 'requests'
-  '';
+  patches = [
+    # Patch failing tests to expect correct Accept-Encoding as generated by Requests
+    (fetchpatch {
+      url = "https://github.com/streamlink/streamlink/commit/ae747a113199c119bced4613d33edcc67a222bb9.patch";
+      includes = [ "tests/test_stream_json.py" ];
+      sha256 = "sha256-KEgyWdh5DNgNktmLSvKQowUQO9p9Q7zP4NbCQJPNgKw=";
+    })
+  ];
 
   meta = with lib; {
     homepage = "https://streamlink.github.io/";