diff --git a/pkgs/development/python-modules/opentelemetry-instrumentation-urllib3/default.nix b/pkgs/development/python-modules/opentelemetry-instrumentation-urllib3/default.nix
new file mode 100644
index 000000000000..ee2b18f00773
--- /dev/null
+++ b/pkgs/development/python-modules/opentelemetry-instrumentation-urllib3/default.nix
@@ -0,0 +1,63 @@
+{
+  buildPythonPackage,
+  opentelemetry-instrumentation,
+
+  # build-system
+  hatchling,
+
+  # dependencies
+  opentelemetry-api,
+  opentelemetry-semantic-conventions,
+  opentelemetry-util-http,
+  wrapt,
+
+  # optional-dependencies
+  urllib3,
+
+  # tests
+  httpretty,
+  opentelemetry-test-utils,
+  pytestCheckHook,
+  respx,
+}:
+
+buildPythonPackage {
+  inherit (opentelemetry-instrumentation) version src;
+  pname = "opentelemetry-instrumentation-urllib3";
+  pyproject = true;
+
+  sourceRoot = "${opentelemetry-instrumentation.src.name}/instrumentation/opentelemetry-instrumentation-urllib3";
+
+  build-system = [ hatchling ];
+
+  dependencies = [
+    opentelemetry-api
+    opentelemetry-instrumentation
+    opentelemetry-semantic-conventions
+    wrapt
+    opentelemetry-util-http
+  ];
+
+  optional-dependencies = {
+    instruments = [
+      urllib3
+    ];
+  };
+
+  pythonImportsCheck = [ "opentelemetry.instrumentation.urllib3" ];
+
+  nativeCheckInputs = [
+    httpretty
+    opentelemetry-test-utils
+    pytestCheckHook
+    respx
+    urllib3
+  ];
+
+  __darwinAllowLocalNetworking = true;
+
+  meta = opentelemetry-instrumentation.meta // {
+    description = "OpenTelemetry urllib3 instrumentation";
+    homepage = "https://github.com/open-telemetry/opentelemetry-python-contrib/blob/main/instrumentation/opentelemetry-instrumentation-urllib3";
+  };
+}
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 463ebbbae93d..06ef62442bfb 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -11236,6 +11236,10 @@ self: super: with self; {
     callPackage ../development/python-modules/opentelemetry-instrumentation-sqlalchemy
       { };
 
+  opentelemetry-instrumentation-urllib3 =
+    callPackage ../development/python-modules/opentelemetry-instrumentation-urllib3
+      { };
+
   opentelemetry-instrumentation-wsgi =
     callPackage ../development/python-modules/opentelemetry-instrumentation-wsgi
       { };