diff --git a/pkgs/development/python-modules/mypy-boto3/default.nix b/pkgs/development/python-modules/mypy-boto3/default.nix
index c253fb15abf7..c49ad4d9f404 100644
--- a/pkgs/development/python-modules/mypy-boto3/default.nix
+++ b/pkgs/development/python-modules/mypy-boto3/default.nix
@@ -322,6 +322,8 @@ rec {
 
   mypy-boto3-groundstation = buildMypyBoto3Package "groundstation" "1.28.36" "sha256-M0HaOpC6zbIaN3VVcM9oE/QzL+me4d5Co5miJGMU3n8=";
 
+  mypy-boto3-guardduty = buildMypyBoto3Package "guardduty" "1.28.53" "sha256-ctMp5+GrKYICPioPi5kB7cnxoP9QFIfbYiDxqf/pPCw=";
+
   mypy-boto3-s3 = buildMypyBoto3Package "s3" "1.28.55" "sha256-sAiAn0SOdAdQEtT8VLAXbeC09JvDjjneMMoOdk63UFY=";
 
   mypy-boto3-xray = buildMypyBoto3Package "xray" "1.28.47" "sha256-1OiTpbaBm2aAls4A7ZaZBNAM8DTRuQcwNKJDq3lOKMY=";
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 233d0d614f56..e29019843654 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -7361,6 +7361,8 @@ self: super: with self; {
 
     mypy-boto3-groundstation
 
+    mypy-boto3-guardduty
+
     mypy-boto3-s3
 
     mypy-boto3-xray