diff --git a/pkgs/development/python-modules/av/default.nix b/pkgs/development/python-modules/av/default.nix
index 7a6da605e338..738d75e59b73 100644
--- a/pkgs/development/python-modules/av/default.nix
+++ b/pkgs/development/python-modules/av/default.nix
@@ -25,14 +25,14 @@
 
 buildPythonPackage rec {
   pname = "av";
-  version = "15.1.0";
+  version = "16.0.1";
   pyproject = true;
 
   src = fetchFromGitHub {
     owner = "PyAV-Org";
     repo = "PyAV";
     tag = "v${version}";
-    hash = "sha256-VeF6Sti1Ide2LchiCuPut/bdbJUv+5eTH2q0YMcniyA=";
+    hash = "sha256-iFKDDOJzCynaqwHIjykfh82diGiuOjWytwU3dq1J9PA=";
   };
 
   build-system = [
@@ -63,10 +63,7 @@ buildPythonPackage rec {
     pytestCheckHook
   ];
 
-  # `__darwinAllowLocalNetworking` doesn’t work for these; not sure why.
-  disabledTestPaths = lib.optionals stdenv.hostPlatform.isDarwin [
-    "tests/test_timeout.py"
-  ];
+  __darwinAllowLocalNetworking = true;
 
   pythonImportsCheck = [
     "av"
diff --git a/pkgs/development/python-modules/av/test-samples.toml b/pkgs/development/python-modules/av/test-samples.toml
index cdc1d93362f1..b20328e97560 100644
--- a/pkgs/development/python-modules/av/test-samples.toml
+++ b/pkgs/development/python-modules/av/test-samples.toml
@@ -1,6 +1,7 @@
 "fate-suite/aac/latm_stereo_to_51.ts" = { url = "http://fate.ffmpeg.org/fate-suite/aac/latm_stereo_to_51.ts", hash = "sha256-lVz0iby2IEUVdwKYamv4HVm8EUGHJS/cWY+QFBMaCBY=" }
 "fate-suite/amv/MTV_high_res_320x240_sample_Penguin_Joke_MTV_from_WMV.amv" = { url = "http://fate.ffmpeg.org/fate-suite/amv/MTV_high_res_320x240_sample_Penguin_Joke_MTV_from_WMV.amv", hash = "sha256-O9YMj0+0bM4YyZNGgkZJL8E2aG+Y3lq8/c+DVht0McI=" }
 "fate-suite/audio-reference/chorusnoise_2ch_44kHz_s16.wav" = { url = "http://fate.ffmpeg.org/fate-suite/audio-reference/chorusnoise_2ch_44kHz_s16.wav", hash = "sha256-KodB5hQkBFtfkI+L7hnkSonPM+IuOCNrTV3Vsy1bvhs=" }
+"fate-suite/h264/extradata-reload-multi-stsd.mov" = { url = "http://fate.ffmpeg.org/fate-suite/h264/extradata-reload-multi-stsd.mov", hash = "sha256-tby9Ttwc4+rVjoaDZ+fqcqCUXSuYjQD/LiLdvhOdlOc=" }
 "fate-suite/h264/interlaced_crop.mp4" = { url = "http://fate.ffmpeg.org/fate-suite/h264/interlaced_crop.mp4", hash = "sha256-SVWWaOcfOp718dvgkpgOWCYoV9Ylomv8MBYzbRqvbBE=" }
 "fate-suite/hap/HAPQA_NoSnappy_127x1.mov" = { url = "http://fate.ffmpeg.org/fate-suite/hap/HAPQA_NoSnappy_127x1.mov", hash = "sha256-WMUqg9o84ki2AIIsGhY8P10KBc3qgCsmljqJXXRHbs8=" }
 "fate-suite/mkv/codec_delay_opus.mkv" = { url = "http://fate.ffmpeg.org/fate-suite/mkv/codec_delay_opus.mkv", hash = "sha256-GanpfRyGKN36NLAa7pZehcM1F2VDCW3g6hhO26vFg1I=" }
@@ -13,5 +14,6 @@
 "fate-suite/qtrle/aletrek-rle.mov" = { url = "http://fate.ffmpeg.org/fate-suite/qtrle/aletrek-rle.mov", hash = "sha256-uXUvVkwuPbfs/rzT896ty3RZfvGoSPj3su+sjLPU09g=" }
 "fate-suite/sub/MovText_capability_tester.mp4" = { url = "http://fate.ffmpeg.org/fate-suite/sub/MovText_capability_tester.mp4", hash = "sha256-Y2uhvfGrZaPebD6ZsJemzpOk+XHX6ukBceVauEit9h8=" }
 "fate-suite/sub/vobsub.sub" = { url = "http://fate.ffmpeg.org/fate-suite/sub/vobsub.sub", hash = "sha256-X2rEMyTlo1xuUlqgx2uvqd2WWhfOCID9fraeGbaFPIs=" }
+"fate-suite/vorbis/vorbis_chapter_extension_demo.ogg" = { url = "http://fate.ffmpeg.org/fate-suite/vorbis/vorbis_chapter_extension_demo.ogg", hash = "sha256-s0kGcghf8Td/bSPy1HOBKaG5bT5zVxFlT1NfQiKHMN8=" }
 "pyav-curated/pexels/time-lapse-video-of-night-sky-857195.mp4" = { url = "https://pyav.org/datasets/pexels/time-lapse-video-of-night-sky-857195.mp4", hash = "sha256-6307VwepfoVNKeGm8WEMZtfowJZ27Hv2fwgp6J/Q0oE=" }
 "pyav-curated/pexels/time-lapse-video-of-sunset-by-the-sea-854400.mp4" = { url = "https://pyav.org/datasets/pexels/time-lapse-video-of-sunset-by-the-sea-854400.mp4", hash = "sha256-2RWphhz5KWPSnJh8ARrC7aPjBa77DJO1Fv0/I4kWxYg=" }