diff --git a/pkgs/development/interpreters/python/hooks/default.nix b/pkgs/development/interpreters/python/hooks/default.nix
index d8b8f661ee2a..8d6c26e7b7f7 100644
--- a/pkgs/development/interpreters/python/hooks/default.nix
+++ b/pkgs/development/interpreters/python/hooks/default.nix
@@ -184,6 +184,12 @@ in
               ] ++ previousPythonAttrs.disabledTestPaths or [ ];
             })
           );
+          disabledTestPaths-item = objprint.overridePythonAttrs (previousPythonAttrs: {
+            pname = "test-pytestCheckHook-disabledTestPaths-item-${previousPythonAttrs.pname}";
+            disabledTestPaths = [
+              "tests/test_basic.py::TestBasic"
+            ] ++ previousPythonAttrs.disabledTestPaths or [ ];
+          });
           disabledTestPaths-glob = objprint.overridePythonAttrs (previousPythonAttrs: {
             pname = "test-pytestCheckHook-disabledTestPaths-glob-${previousPythonAttrs.pname}";
             disabledTestPaths = [
diff --git a/pkgs/development/interpreters/python/hooks/pytest-check-hook.sh b/pkgs/development/interpreters/python/hooks/pytest-check-hook.sh
index 1696b180b3f6..50757ec5b7ae 100644
--- a/pkgs/development/interpreters/python/hooks/pytest-check-hook.sh
+++ b/pkgs/development/interpreters/python/hooks/pytest-check-hook.sh
@@ -13,8 +13,11 @@ function pytestCheckPhase() {
     local -a _pathsArray=()
     concatTo _pathsArray disabledTestPaths
     for path in "${_pathsArray[@]}"; do
-        # Check if every path glob matches at least one path
-        @pythonCheckInterpreter@ - "$path" <<EOF
+        if [[ "$path" =~ "::" ]]; then
+            flagsArray+=("--deselect=$path")
+        else
+            # Check if every path glob matches at least one path
+            @pythonCheckInterpreter@ - "$path" <<EOF
 import glob
 import sys
 path_glob=sys.argv[1]
@@ -23,7 +26,8 @@ if not len(path_glob):
 if next(glob.iglob(path_glob), None) is None:
     sys.exit('Disabled tests path glob "{}" does not match any paths. Aborting'.format(path_glob))
 EOF
-        flagsArray+=("--ignore-glob=$path")
+            flagsArray+=("--ignore-glob=$path")
+        fi
     done
 
     if [ -n "${disabledTests[*]-}" ]; then