diff --git a/pkgs/development/python-modules/mypy-boto3/default.nix b/pkgs/development/python-modules/mypy-boto3/default.nix
index d38665901f1c..6dee6bca151c 100644
--- a/pkgs/development/python-modules/mypy-boto3/default.nix
+++ b/pkgs/development/python-modules/mypy-boto3/default.nix
@@ -878,8 +878,8 @@ in
       "sha256-bGxwx1LeiPWn6gz4Yqu6pIQ7sMgCro5W4oO/Wk72gok=";
 
   mypy-boto3-mediastore =
-    buildMypyBoto3Package "mediastore" "1.42.3"
-      "sha256-BieG4cimqiiVqdP7/J1tsCc5YQlVEbiA5bOYCvUBspY=";
+    buildMypyBoto3Package "mediastore" "1.43.0"
+      "sha256-tT1iRnm3gOaY8clsRshI9NL1FF4aHlBhWyJMi/7HpE8=";
 
   mypy-boto3-mediastore-data =
     buildMypyBoto3Package "mediastore-data" "1.42.3"