diff --git a/pkgs/development/python-modules/mypy-boto3/default.nix b/pkgs/development/python-modules/mypy-boto3/default.nix
index 373f1af3423f..255764d7a3a0 100644
--- a/pkgs/development/python-modules/mypy-boto3/default.nix
+++ b/pkgs/development/python-modules/mypy-boto3/default.nix
@@ -443,8 +443,8 @@ in
       "sha256-dXNkOcMonYrBh4yzeubd+v3mW42s9XpmpfvgbtgoJgY=";
 
   mypy-boto3-ec2 =
-    buildMypyBoto3Package "ec2" "1.43.26"
-      "sha256-IGl4y8hDpdsqKcky6UgukfDlnSjbyYsa69sPiYZYcN4=";
+    buildMypyBoto3Package "ec2" "1.43.27"
+      "sha256-Cz3DhKTxDSluH14aqeY+lPuCGTEOoC0DJp7A3RCP7Gg=";
 
   mypy-boto3-ec2-instance-connect =
     buildMypyBoto3Package "ec2-instance-connect" "1.43.0"